【摘要】 染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing, ATAC-seq)是2013年在人类免疫细胞中建立的用于研究表观遗传调控的重要技术。该技术已在人类及小鼠等模式动物的基因组调控元件鉴定、转录因子结合位点识别及转录调控机制的解析等研究领域发挥了重要作用。然而，ATAC-seq技术在植物领域中的研究应用还处于起步阶段，相关研究主要集中在拟南芥和水稻等模式植物中。笔者主要概述了ATAC-seq技术在植物中的应用，包括染色质可及性图谱绘制、抗逆机制解析、表观修饰鉴定及调控元件识别等领域的研究进展，并进一步阐述了ATAC-seq在木本植物中的应用潜力，以推动ATAC-seq技术在木本植物表观基因组学研究中的应用。更多还原

【Abstract】 Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq) is an important technique established in 2013 in human immune cells to study epigenetic regulation. This technique has been used in the identification of genomic regulatory elements, binding sites of transcription factors and analysis of transcriptional regulation mechanism in human and mouse model animals. However, the application of ATAC-seq technique in plants is still in its infancy, and related studies mainly focus on model plants such as Arabidopsis and rice. In this review, we discuss the application of ATAC-seq in plants, including chromatin accessibility sites mapping, stress resistance mechanisms revealing, epigenetic modification and regulatory elements identification. Furthermore, we look at the prospects of applying ATAC-seq in woody plant epigenomics so as to promote its application in woody plant epigenomics research.更多还原