【摘要】 一氧化氮（NO）是一种具有高细菌抑制率的气体信号分子，被广泛用于生物医学治疗当中，但是目前还存在如NO供体材料生物相容性差、NO负载量低以及控释缓释效果不佳等问题。以我国盛产的海藻多糖海藻酸钠（SA）为原料，制备了巯基化海藻酸钠（SA-SH）和NO供体S-亚硝基海藻酸钠（SA-SNO），并以不同比例复合SA-SH与SA-SNO制备了原位成型水凝胶。结果表明随着SA-SNO含量的增加，NO负载量显著增加；释放曲线表明该凝胶具有较长的释放周期，5 d后NO释放量高达30.12～44.32μmol·g^-1，总释放量为152～264μmol·mg^-1。抗菌实验表明水凝胶对金黄色葡萄球菌（Staphylococcus aureus）、大肠杆菌（Escherichia coli）具有显著抑制作用。综上所述，研究制备的水凝胶具有作为伤口抗菌敷料的应用潜力。更多还原

【Abstract】 Nitric oxide（NO） is a gas signaling molecule with high bacterial inhibition rate, which has been widely used in biomedical treatment. However, there are still problems such as poor biocompatibility of NO donor materials, low NO load, poor control release and slow-release effect. In this study, we used an aquatic polysaccharide material, sodium alginate（SA）, to prepare sulfhydryl sodium alginate（SA-SH） and NO donor S-nitroso sodium alginate（SA-SNO）, and prepared in-situ formed hydrogel by using different ratios of SA-SH and SA-SNO. The results show that the NO loading in the hydrogels increased with the increasing content of SA-SNO, and the release curve indicates that the gel possessed long-lasting release effects, wherein the releasing amount of NO arrived to 30.12-44.32 μmol·g^-1 even after 5 d and the total release amount was 152-264 μmol·g^-1. Additionally, antibacterial experiments show that the hydrogel had a significant inhibitory effect on Staphylococcus aureus and Escherichia coli. In summary, the composite hydrogel has the potential application as an anti-bacterial wound dressing.更多还原