【摘要】 针对富含优质植物蛋白的“药食两用”桑叶，探索一种有更高的蛋白提取率的工艺方法。以桑叶粉为原料，采用单因素筛选试验和正交设计方法，研究了超声波-纤维素酶联合辅助提取法提取桑叶蛋白质的工艺。获得桑叶蛋白质提取工艺的最佳参数为提取料液比1∶28 (g∶m L)提取溶剂是质量分数为0.9%的Na Cl溶液，150 W的超声波处理30 min,50℃水浴浸提50 min，残渣中添加4.5%纤维素酶，在50℃下酶解55 min，酸沉淀蛋白质的p H值为2.0；按以上参数进行桑叶蛋白质的提取，其提取率可达16.06%。该联合辅助提取法的桑叶蛋白质得率比单独使用其中一种辅助方法或其他的方法的提取率要高得多，至少高出56.99%；该提取工艺大大提高了提取率，可为同行及进一步产业化的研究提供参考。更多还原

【Abstract】 Aiming at the mulberry leaves which are rich in high-quality plant protein,a technology with higher extraction rate of protein was explored. Using mulberry leaf powder as raw material,the extraction technology of mulberry leaf protein by ultrasonic-cellulase assisted extraction was studied by single factor screening test and orthogonal design. The optimum parameters of extracting mulberry leaf protein were as follows:the ratio of extracting material to liquid was 1∶28(g∶m L),the extracting solvent was 0.9% Na Cl solution,150 W ultrasonic treatment for 30 min,50 min water bath extraction in 50 ℃,4.5%cellulase added to the precipitate and enzymolysis at 50 ℃ for 55 min,the p H value of acid precipitated protein was 2.0. According to these parameters,mulberry leaf protein extraction,the yield could reach 16.06%. The yield of mulberry leaf protein by the combined-assisted extraction method was much higher than that by one of the assistant methods alone or other methods,at least 56.99% higher. The extraction process greatly improved the extraction yield,which could provide a reference for the research of peers and further industrialization.更多还原