【摘要】 【目的】探究哈克尼西棉不育胞质（CMS-D2）和三裂棉不育胞质（CMS-D8）对 ATP 和 H₂O₂含量的影响。【方法 】选 用 CMS-D2 和 CMS-D8 两套 “三 系 ”（不 育 系 、保 持 系 及 其 恢 复 系 ）材料 ，测定 其 不 同发 育 时 期 的 叶 片和花蕾中 ATP 和 H₂O₂含量；并结合已有研究测定候选不育基因 orf610a 超表达拟南芥植株中 ATP 和 H₂O₂含量；通过实时荧光定量聚合酶链式反应分析 ATP 合成相关基因在不育系、保持系以及转基因拟南芥花蕾中的表达量。【结果】与其保持系和恢复系相比，CMS-D2 不育系叶片在蕾期和铃期均表现为 ATP 含量极显著降低、H₂O₂含量极显著升高；CMS-D8 不育系叶片在蕾期表现为 ATP 含量极显著降低、H₂O₂含量极显著升高，在铃期表现为 ATP 含量极显著升高；CMS-D2 不育系 3 mm、4 mm 花蕾中 ATP 含量极显著降低，4 mm 花蕾中H₂O₂含量极显著上升；CMS-D8 不育系 5 mm、6 mm 花蕾中 ATP 含量极显著降低。 与其保持系相比，CMS-D2和 CMS-D8 不育系花蕾中，多个 ATP 合成相关基因的表达量极显著降低。 与野生型材料相比，orf610a 超表达拟南芥角果和花蕾中的 H₂O₂含量显著升高，ATP 含量极显著降低， 且大多数 ATP 合成相关基因均极显著下调表达。 【结论】不育胞质会对棉花不同发育时期的叶片和花蕾中 ATP 和 H₂O₂含量产生一定的影响，二者动态平衡的破坏可能会影响花粉的正常发育。更多还原

【Abstract】 Objective] This research aims to investigate the effects of male sterile cytoplasm of Gossypium harknessii （CMS-D2）and Gossypium trilobum （CMS-D8） on ATP and H₂O₂contents. [Method] The contents of ATP and H₂O₂in leaves and flower buds of CMS-D2 and CMS-D8 "three lines" （sterile line, maintainer line, and restorer line） at different developmental stages were determined, and combined with the subject of existing research, ATP and H₂O₂contents in Arabidopsis plants overexpressing the sterile candidate gene orf610a were determined. The expression levels of ATP synthesis-related genes in the flower buds of sterile lines, maintainer lines and transgenic Arabidopsis were analyzed by quantitative real-time polymerase chain reaction（qRT-PCR）. [Result] During the development of cotton, compared with its maintainer line and restorer line, the leaves of CMS-D2 sterile line showed an extremely significant decrease in ATP content and an extremely significant increase in H₂O₂content at both the bud stage and boll stage; the leaves of CMS-D8 sterile line showed an extremely significant decrease in ATP content and an extremely significant increase in H₂O₂content at the bud stage, and an extremely significant increase in ATP content at the boll stage. The ATP content in 3 mm and 4 mm flower buds of CMS-D2 sterile line was extremely significantly decreased, and the H₂O₂content in 4 mm flower buds was extremely significantly increased; the ATP contents in 5 mm and 6 mm flower buds of CMS-D8 sterile line were extremely significantly decreased. Compared with the maintainer lines, the expression levels of several ATP synthesis-related genes in the flower buds of the CMS-D2 and CMS-D8 sterile lines were extremely significantly reduced . Compared with wild-type Arabidopsis , the H₂O₂contents in siliques and flower buds of orf610aoverexpressed Arabidopsis were significantly increased, and the ATP content were extremely significantly decreased, and most ATP synthesis-related genes were extremely significantly down-regulated. [Conclusion] The male sterile cytoplasm have certain effects on the contents of ATP and H₂O₂in leaves and flower buds at different developmental stages of cotton, and the disruption of the dynamic balance may affect the development of pollen.更多还原