【摘要】 对西方蜜蜂在采蜜过程中细菌的变化进行研究，通过传统纯化培养及1 6S r DNA基因序列鉴定，结果表明：花蜜、蜜囊到0d蜂蜜细菌数量从1 0 CFU-1 06CFU，以解淀粉芽孢杆菌（Bacillus amyloliquefaciens）为优势菌，所分离鉴定的菌株提交Gen Bank比对后，其相似度不低于99%。细菌系统发育分析包括Ⅰ红螺菌、Ⅱ柄杆菌、Ⅸ芽孢杆菌等9个簇。更多还原

【Abstract】 Changes of bacterial diversity in the process of collection of Apis mellifera were studied. Bacteria were isolated and cultivated to obtain pure isolates in nectar, 0d honey and honey stomach of Apis mellifera. The isolates were compared and a phylogeny constructed analysis based on 1 6S r DNA sequences amplification and sequencing. Bacillus amyloliquefaciens was the dominant bacteria from 1 0 CFU to 1 06CFUin nectar honey stomach and 0d Immature honey. Isolates comparison with Gen Bank accession numbers, the similarity is not less than 99%.Phylogenetic analysis 9clusters including Brevundimonas、Bacillus and Agrobacterium and so on.更多还原