【摘要】 [目的 ]探析南方竹区重要地下害虫筛胸梳爪叩甲幼虫定位寄主的行为及化学机制，为竹林金针虫的高效监测及防控提供科学依据。[方法 ]利用双向选择仪测定筛胸梳爪叩甲幼虫对竹笋和CO₂释放剂颗粒的行为反应。采用固相微萃取气质联用法（SPME-GC-MS）收集鉴定雷竹鲜笋的挥发性组分，并测定其相对含量。[结果 ]竹笋和CO₂释放剂均能显著引发筛胸梳爪叩甲幼虫定向运动。筛胸梳爪叩甲幼虫对不同质量鲜笋的取食选择无显著差异（P>0.05），而不同浓度的CO₂释放剂对筛胸梳爪叩甲幼虫的定向行为有显著影响（P<0.05）。在竹笋和CO₂释放剂之间，筛胸梳爪叩甲幼虫显著偏好竹笋（P<0.05）。收集鉴定雷竹笋挥发物17种，相对含量较高为烯烃类化合物（66.62%）和倍半萜（27.98%），倍半萜中以α-葎草烯含量最高。[结论 ]筛胸梳爪叩甲幼虫对新鲜雷竹笋和CO₂释放剂颗粒均有显著趋向性，筛胸梳爪叩甲幼虫更偏好新鲜竹笋，相比CO2释放剂，竹笋萌发时所释放的挥发物对竹林金针虫搜寻寄主更为有效。本研究分离鉴定7种可能对筛胸梳爪叩甲幼虫具有吸引作用的萜烯类化合物，为竹林金针虫新型防控技术研发提供了重要依据。更多还原

【Abstract】 [Objective] To understand the mechanism of food foraging below ground of Melanotus cribricollis larva which is the dominant and most destructive soil-dwelling insect of bamboo shoots in south China and provide important basic information for the development of effective monitor and control techniques.[Method] We tested the orientation behavior of M. cribricollis larvae towards bamboo shoot and CO₂-releasing capsules by using a dual-choice olfactometer. The volatiles emitted from Phyllostachys violascens shoot were tentatively identified by solid phase micro-extraction（SPME） coupled with GC-MS, and their relative content were determined as well. [Result] Both bamboo shoot and CO₂ source could significantly induce the directional movement of the larvae of M. cribricollis. There was no significant difference in the larval behavior among different weights of bamboo shoots（P>0.05）, whiel larval orientation behavior was significantly correlated with CO₂ concentration（P<0.05）. Between bamboo and CO₂-releasing capsules, wireworms preferred significantly to the bamboo shoot（P<0.05）. A total of 17 compounds emitted from Ph.violascens shoot were tentatively identified. The higher relative contents were olefins（66.62%） and sesquiterpenes（27.98%）, and the sesquiterpene compound with the highest content was α-humulene.[Conclusion] M. cribricollis larvae shows significant stronger orientation towards bamboo shoot and CO₂source, and prefers bamboo shoot significantly. Compared with CO₂ source, the volatile organic compounds（VOCs） emitted from bamboo shoot show to be more effective in food foraging of bamboo shoot wireworm. In addition, 7 terpene compounds with potential attraction to wireworms are identified in this study, which provids important basic information for the development of attract-and-kill formulations for the control of wireworms.更多还原