【摘要】 目的 观察“三穴五针法”针刺对哮喘小鼠肺组织中铁代谢相关分子表达的影响。方法 选用SPF级别雌性BALB/c小鼠40只，随机分为正常对照组、哮喘模型组、针刺组、假针刺组，每组10只。采用卵清蛋白(OVA)致敏法复制哮喘小鼠模型。针刺组给予针刺大椎、双侧风门、双侧肺俞，假针刺组给予针刺双侧环跳穴，1次/d,连续3 d。采用有创小动物肺功能仪检测小鼠气道阻力及动态肺顺应性以评估小鼠肺功能；采用HE和PAS染色法检查小鼠肺组织的病理学变化；采用Western blot及qRT-PCR检测小鼠肺组织中铁代谢相关分子的蛋白表达水平及mRNA水平。结果 与正常对照组小鼠比较，哮喘小鼠的气道阻力增加，肺顺应性降低；肺组织中炎症细胞浸润和杯状细胞化生明显增多；肺组织中铁转入相关蛋白TFR1、 DMT1和Ferritin表达水平增高，铁转出蛋白FPN的表达水平降低，铁代谢相关蛋白的编码基因TFR1、TFR2、ZIP14、FTL、FTH、NRAMP1、HEPC1的mRNA水平显著增高。与哮喘模型组比较，给予“三穴五针法”针刺干预后，可显著改善哮喘小鼠肺功能，减轻哮喘小鼠肺组织中炎症细胞浸润和杯状细胞化生，并显著降低TFR1、 DMT1和Ferritin表达水平，显著升高FPN的表达水平，小鼠肺组织中的TFR1、TFR2、ZIP14、FTL、FTH、NRAMP1、HEPC1的mRNA表过水平显著降低。结论 “三穴五针法”针刺可改善哮喘小鼠肺功能，减轻肺部炎症，其机制可能与通过调控肺组织中铁代谢相关分子的表达来调节肺组织铁代谢紊乱相关。更多还原

【Abstract】 Objective To observe the effects of “three points and five needles” acupuncture on the expression of iron metabolism-related molecules in the lung tissue of asthmatic mice. Methods Forty SPF female BALB/c mice were randomly divided into normal control group, asthma model group, acupuncture group and sham acupuncture group, with 10 mice in each group. The mouse model of asthma was induced by sensitization with ovalbumin(OVA). The acupuncture group was given acupuncture at Dazhui, bilateral damper and bilateral Feishu points, and the sham acupuncture group was given acupuncture at bilateral Huantiao points. The acupuncture was performed once a day for 3 consecutive days. The invasive small animal lung function instrument was used to detect the airway resistance and dynamic lung compliance in mice to evaluate the lung function of mice. The pathological changes of lung tissue were examined by HE and PAS staining. Western blot and qRT-PCR were used to detect the levels of protein and mRNA of iron metabolism-related molecules in lung tissue of mice. Results Compared with mice in the normal control group, the airway resistance of asthmatic mice increased and the lung compliance decreased; inflammatory cell infiltration and goblet cell metaplasia increased significantly in lung tissue of asthmatic mice; the expression level of iron transfer-related proteins TFR1, DMT1 and Ferritin in lung tissue increased, the expression level of iron transfer out protein FPN decreased, and the mRNA level of iron metabolism-related protein encoding genes TFR1, TFR2, ZIP14, FTL, FTH, NRAMP1, HEPC1 increased significantly. Compared with the asthma model group, the “three points and five needles” acupuncture intervention could significantly improve the lung function of asthma mice, reduce the infiltration of inflammatory cells and goblet cell metaplasia in the lung tissue of asthma mice, reduce significantly the expression levels of TFR1, DMT1 and Ferritin, increase significantly the expression level of FPN, and reduce significantly the mRNA levels of TFR1, TFR2, ZIP14, FTL, FTH, NRAMP1, HEPC1 in the lung tissue of asthmatic mice. Conclusion “Three point five needle method” acupuncture can improve lung function and reduce lung inflammation in asthmatic mice. Its mechanism may be related to regulating the disorder of iron metabolism in lung tissue by regulating the expression of iron metabolism-related molecules in lung tissue.更多还原