【摘要】 为探究发酵对金针菇多酚提取、纯化及抗油脂氧化能力的影响,以金针菇和发酵金针菇为原料,探讨以超临界CO₂流体萃取法提取金针菇多酚的工艺条件,以及以柱层析法纯化多酚的工艺条件,并测定金针菇多酚（FVP）和发酵金针菇多酚（FFVP）的抗油脂氧化能力。结果表明:金针菇多酚的最佳提取条件为萃取时间140 min、萃取压力30 MPa、萃取温度60℃和CO₂流量20 L/h,FVP和FFVP的提取得率分别为0.89%和0.95%;金针菇多酚的最佳纯化条件为上样量4 BV、质量浓度3.5 mg/mL、pH4.5和乙醇体积分数60%,FVP和FFVP的纯度分别为85.29%和85.74%;在同等条件下,FFVP的抗油脂氧化能力高于FVP。更多还原

【Abstract】 The effect of fermentation on the extraction and purification of polyphenols from Flammulina velutipes and their anti-lipid oxidation ability were explored.Using Flammulina velutipes and fermented Flammulina velutipes as raw materials, the extraction conditions of Flammulina velutipes polyphenols by supercritical CO₂ fluid extraction and the purification conditions of polyphenols by column chromatography were studied.The anti-lipid oxidation ability of Flammulina velutipes polyphenols （FVP） and fermented Flammulina velutipes polyphenols （FFVP） were determined.The results showed that the optimal extraction conditions of Flammulina velutipes polyphenols were as follows: extraction time 140 min, extraction pressure 30 MPa, extraction temperature 60 ℃ and CO₂ flow 20 L/h.The extraction yield of FVP and FFVP were 0.89 % and 0.95 %, respectively.The optimal purification conditions of Flammulina velutipes polyphenols were as follows: loading amount 4 BV, mass concentration 3.5 mg/mL, pH 4.5 and ethanol volume fraction 60 %.The purities of FVP and FFVP were 85.29 % and 85.74 %, respectively.Under the same conditions, FFVP had higher anti-lipid oxidation ability than FVP.更多还原