【摘要】 内生菌对水稻的生长、发育及产量等性状具有重要影响。然而，何种消毒方式既能达到很好的消毒效果又对水稻内生菌的影响最小尚不清楚。研究以水稻不育系乐3A种子为研究材料，通过比较常见的消毒试剂以及不同浓度次氯酸钠对其表面消毒的效果及对内生菌的影响，筛选出既能高效灭菌又不会破坏水稻种子内生菌的最优表面消毒方法。实验结果表明，次氯酸钠试剂较其他试剂消毒效果最好，更重要的是，用浓度为5.5%的次氯酸钠溶液对水稻种子进行消毒处理15s时，对水稻内生菌破坏越小。研究结果为探究水稻种子与内生菌之间的关系奠定了实验基础。更多还原

【Abstract】 Endophytes play an important role in the growth, development and yield of rice. However, it is not clear which disinfection method can achieve the highest disinfection effect and the least impact on rice endophytes. In this study, we used the rice sterile line Le 3A seeds as the research material, and compared the disinfectant efficiency of common agents and the effect of different concentrations of NaClO on endophytic bacteria, to screen out the best methods to sterilize rice seed without destroying the internal bacteria. The results showed that NaClO has the best disinfection effect than other reagents. More importantly, we found that disinfect the surface of rice seeds with 5.5% NaClO for 15s, can achieve the best disinfectant efficiency, with the less damage to the endophytes of rice. The results of this study will lay an experimental foundation for exploring the relationship between rice seeds and endophytes.更多还原