【摘要】 目的 探讨miR-155通过调节IL-21受体参与特应性皮炎的发病机制。方法 选取2018年1月至2020年5月急性特应性皮炎患者110例为观察组，健康体检者102例为对照组，测定两组miR-155、IL-21R、IL-4、hs-CRP、TNF-α、CD44、Th21、IL-21水平。设Jurkat组、miR-155 mimics组（Jurkat细胞经miR-155过表达载体转染）、miR-155 inhibitor组（Jurkat细胞经miR-155低表达载体转染）。测定各组细胞增殖、凋亡水、miR-155、IL-21R mRNA和蛋白水平。结果 观察组miR-155、IL-21R mRNA水平、IL-4、hs-CRP、TNF-α、CD44、Th21、IL-21高于对照组（P<0.05）。miR-155与TNF-α、IL-4、IL-21、TH21、IL-21R mRNA、hs-CRP、CD44正相关（P<0.05）。与Jurkat组比较，miR-155 NC组各指标差异无统计学意义（P>0.05）；与Jurkat组比较，miR-155 mimics组OD值、存活率升高，凋亡率降低（P<0.05）,miR-155 inhibitor组OD值、存活率降低，凋亡率升高（P<0.05）；与miR-155 mimics组比较，miR-155inhibitor组OD值、存活率降低，凋亡率升高（P<0.05）。与Jurkat组比较，miR-155 mimics组细胞miR-155 mRNA、IL-21R mRNA和蛋白升高，miR-155 inhibitor组细胞miR-155 mRNA、IL-21R mRNA和蛋白降低（P<0.05）；与miR-155 mimics组比较，miR-155 inhibitor组细胞miR-155 mRNA、IL-21R mRNA和蛋白降低（P<0.05）。结论 特应性皮炎患者miR-155、IL-21R表达水平升高；miR-155可能通过促进IL-21R的表达进而参与特应性皮炎的发病。更多还原

【Abstract】 Objective To investigate the miR-155 participates in the pathogenesis of atopic dermatitis by regulating IL-21 receptor.Methods 110 patients with acute atopic dermatitis from January 2018 to May 2020 in our hospital were selected as the case group and 102 healthy subjects as the control group. The levels of miR-155,IL-21R,IL-4,hs-CRP,TNF-α,CD44,Th21,IL-21 in two groups were measured. Jurkat group,miR-155mimics group(Jurkat cells were transfected with mi R-155 overexpression vector)and mi R-155 inhibitor group(Jurkat cells were transfected with mi R-155low expression vector)were set up. MTT method and flow cytometry were used to determine the cell proliferation and apoptosis levels in Jurkat cells of each group,and RT-PCR and Western-blot methods were used to determine the mRNA and protein levels of miR-155 and IL-21R. Results The levels of miR-155,IL-21R mRNA,IL-4,hs-CRP,TNF-α,CD44,Th21 and IL-21 in the case group were higher than those in the control group(P<0.05).The miR-155 was positively correlated with TNF-α,IL-4,IL-21,TH21,IL-21R mRNA,hs-CRP and CD44(P<0.05). Compared with the Jurkat group,there were no significant changes in the all indexes in the miR-155 NC group(P>0.05). Compared with the Jurkat group,the OD value and survival rate of the miR-155 mimics group were increased,the apoptosis rate decreased(P<0.05),the OD value and survival rate in the miR-155 inhibitor group decreased,and the apoptosis rate increased(P<0.05). Compared with miR-155 mimics group,the OD value and survival rate of miR-155 inhibitor group were decreased,and the apoptosis rate increased(P<0.05). Compared with the Jurkat group,the miR-155 mRNA,IL-21R mRNA and protein in the miR-155 mimics group were increased,and the miR-155 mRNA,IL-21R mRNA and protein decreased in the miR-155 inhibitor group(P<0.05).Compared with the mi R-155 mimics group,the mi R-155 m RNA,IL-21R m RNA and protein of the mi R-155 inhibitor group were decreased(P<0.05).Conclusion The expression levels of miR-155 and IL-21R in patients with atopic dermatitis are elevated. The miR-155 may participate in the pathogenesis of atopic dermatitis by promoting the expression of IL-21R.更多还原