【摘要】 目的 探索和评价不同方法建立的小鼠炎症性肠病合并艰难梭菌感染的模型。方法 首先采用口服葡聚糖硫酸钠(DSS)水溶液建立小鼠炎症性肠病模型，然后于炎症性肠病急性期和恢复期，分别腹腔注射克林霉素或头孢哌酮诱导小鼠感染艰难梭菌，观察感染后每组小鼠的体重、疾病活动度、生存率和组织病理变化。结果 炎症性肠病急性期、恢复期小鼠采用克林霉素诱导合并艰难梭菌感染时，均在感染后第3天体重下降最明显，疾病活动指数评分最高，小鼠生存率分别为25%和0，死亡小鼠组织病理学评分差异无统计学意义(P>0.05)。头孢哌酮诱导的小鼠主要在感染后第1、2天体重下降明显、疾病活动指数评分较高，小鼠生存率分别为62.5%和87.5%，组织病理学评分也无显著差异，疾病严重程度均弱于相应克林霉素诱导的小鼠。结论 相较于头孢哌酮，采用腹腔注射克林霉素的方法建立炎症性肠病急性期和恢复期小鼠合并艰难梭菌感染模型疾病更为严重，生存率更低。更多还原

【Abstract】 Objective To explore and evaluate two different methods for establishing mouse model of comorbidity of inflammatory bowel disease (IBD) and Clostridioides difficile infection (CDI).Methods We firstly used dextran sulphate sodium (DSS) solution to establish mouse IBD model.Then the successful mouse IBD model was treated with clindamycin and cefoperazone by intraperitoneal injection at acute and recovery phase of disease respectively to induce CDI.Body weight,disease activity,survival rate,and histopathological scores of colon tissues were monitored after CDI.Results After treatment with clindamycin at either acute or recovery phase of IBD,the most significant weight loss and the highest disease activity index of mice were observed on Day 3 after CDI.The survival rate was 25% and 0,respectively.The histopathological scores of colon tissues did not show significant difference among the dead mice.After treatment with cefoperazone at either acute or recovery phase of IBD,the significant weight loss and disease activity were observed on Day 1 or Day 2 after CDI.The survival rate was 62.5% and 87.5%,respectively.The histopathological scores of colon tissues did not show significant difference.Cefoperazone-treated mice demonstrated less severe disease than corresponding clindamycin-treated mice.Conclusions Compared with the CDI model induced by cefoperazone,the clindamycin-induced CDI model in IBD mouse tends to have more severe disease and lower survival rate.更多还原