【摘要】 目的:建立参芍胶囊中人参皂甙Rg₁含量测定的高效液相色谱方法。方法:用Hypersil-ODS柱（4.6 mm×150mm,5μm）分离,甲醇-水（体积比50∶50）洗脱,流速1.0mL/min,检测波长设置为203nm。结果:人参皂甙Rg₁在2.6～26μg范围内具有良好的线性关系,其线性方程为Y =231896X +44038（n =10,r=0.9994）,平均加样回收率为99.84%,（RSD=0.91%n=6）。结论:用该法测定人参皂甙Rg₁的含量,线性关系良好、精密度较好、准确度高、稳定性好,可用于参芍胶囊中 Rg₁质量控制。更多还原

【Abstract】 Objective:AHPLC method for the determination of ginsenoside Rg₁ in Shenshao capsules was established in this paper.Methods:Separationwas completed in Hypersil-ODS column（4.6 mm×150 mm,5 μm）.Methanol-water（50 : 50） was used as mobile phase.The flow rate was 1.0 mL/min.Detection wavelength was set at 203 nm.Results:Linear relationship was good（r=0.999 4） in the range of 2.6～26 μg with equation of Y=231 896 X+44 038（n=10）.The average recovery was 99.84% RSD=0.91 %,（n=6）.Conclusion:This method was good linearity,accurate,precise and stabile,which was suitable for the quality control of Rg₁ in Shenshao capsules.更多还原