【摘要】 【目的】茶尺蠖核型多角体病毒EcobNPV是一种重要的昆虫病原微生物，可侵染茶尺蠖Ectropis obliqua和灰茶尺蠖Ectropisgrisescens2种宿主。为探明茶尺蠖核型多角体病毒制剂的田间应用技术，对影响防效的3个因子喷施方式、剂量和虫龄进行研究。【方法】采用植保无人机和人工喷施2种喷施方式，比较茶核·苏云菌悬浮剂（1×104 PIB·2 000 IU/μL）防治灰茶尺蠖的效果；设置75、150和375 mL/667 m~23种剂量，比较茶核·苏云菌悬浮剂对灰茶尺蠖的防治效果；并比较其对灰茶尺蠖1龄、2龄和3龄不同虫龄的防治效果。【结果】在相同剂量下，用植保无人机和人工喷施病毒制剂对灰茶尺蠖的总防效分别为69.0%和67.6%，两者无显著差异；在75、150和375 mL/667 m~2 3种剂量下病毒制剂对灰茶尺蠖的防效差异显著，其中375 mL/667 m~2剂量下的防效最高，150 mL/667 m~2其次，150 mL/667 m~2和375 mL/667 m~2剂量对灰茶尺蠖的总防效均在90%以上；病毒制剂对灰茶尺蠖1龄、2龄和3龄幼虫的防效分别为93.3%、78.7%和67.8%，对1龄幼虫的防效显著高于3龄。【结论】茶核·苏云菌悬浮剂防治灰茶尺蠖可选用植保无人机喷施，剂量可选用150-375 mL/667 m~2，以1龄幼虫期喷施防效为最佳。更多还原

【Abstract】 [Objectives] To investigate techniques for using Ectropis obliqua nucleopolyhedrovirus （Ecob NPV） preparations,a pathogen that can infect both Ectropis obliqua and Ectropis grisescens, to control these pests. [Methods] The effects of different application methods （drone and sprayer）, larval instar (1^st, 2^nd and 3^rd instar larvae) and dosages （75, 150 and375 m L/667 m²） on the effectiveness of Ecob NPV preparations containing both 1×10⁴ PIB/μL Ecob NPV and 2 000 IU/μL Bacillus thuringensis, were tested in field trials. [Results] There was no significant difference in the effectiveness of Ecob NPV preparations of the same dosage applied with either a drone （69.0%） or sprayer （67.6%）. The optimal dose of Ecob NPV preparations was 375 m L/667 m², followed by 150 m L/667 m²; the effectiveness of both doses exceeded 90%. The effectiveness of Ecob NPV preparations on 1^st, 2^nd and 3^rd instar larvae was 93.3%, 78.7% and 67.8%, respectively, and its effectiveness on 1^st instar larvae was significantly higher than that on 3^rd instar larvae. [Conclusion] Ecob NPV preparations can be applied by drones to control tea geometrids. First instar larvae are most susceptible to Ecob NPV preparations and the optimal dose is 150-375 m L/667 m².更多还原