【摘要】 目的 分析2020年河南省济源市报告的一起食源性疾病暴发事件，查明事件原因，制定有效的食源性疾病预防控制措施。方法 采用现场流行病学方法对此次食源性疾病暴发事件开展病例调查；采集可疑剩余食品及患者呕吐物和粪便等样本，以传统方法进行食源性致病菌的分离鉴定，采用多重荧光PCR法对分离到的致病性大肠埃希菌进行毒力基因检测，并用脉冲场凝胶电泳（PFGE）对菌株聚类分析。结果 调查病例79例，罹患率25.08%(79/315)，临床表现以腹痛、恶心和腹泻为主；采集标本22份（食品样本10份，患者标本12份），从五香牛肉、大刀耳片和3份粪便共计5份样本中检出致病性大肠埃希菌，检出率22.73%；分离菌株18株，经多重荧光PCR检测，均显示肠聚集性大肠埃希菌（EAEC）毒力基因阳性；所有菌株经PFGE分析显示同源性>85%。结论根据现场流行病学调查和实验室检测结果，可判断该食源性疾病是因食用被EAEC污染的食品引起；多重荧光PCR法和PFGE分型技术可作为致病性大肠埃希氏菌检测的重要参考依据。更多还原

【Abstract】 Objective This study aims to analyze an outbreak of food-borne disease reported in Jiyuan city,Henan Province in 2020,and to find out the causes of this outbreak and formulate effective measures for the prevention and control of food-borne diseases. Methods Field epidemiological method was used to investigate the outbreak of food-borne diseases. Samples of suspect surplus food and patients’ vomitus and faeces were collected for isolation and identification of food-borne pathogens by traditional methods. Multiple fluorescent PCR was utilized to detect the pathogenic Escherichia coli. Cluster analysis of strains was performed by pulsed field gel electrophoresis(PFGE). Results A total of 79 cases were investigated,with anattack rate of 25.08%(79/315). The main clinical manifestations were abdominal pain,nauseaand diarrhea. Twenty-two samples were collected,including 10 food samples and 12 patient samples. Pathogenic Escherichia coli was detected in 5 samples,and the detection rate was 22.73%. Eighteen strains were isolated and showed positive virulence genes of enteroaggregative Escherichia coli(EAEC). PFGE analysis showed that the homology of all strains was more than 85%. Conclusion Based on the results of field epidemiological investigation and laboratory testing,it can be concluded that the food-borne disease was caused by eating food contaminated with EAEC,and the multiplex PCR and PFGE typing can be used as important reference for the detection of pathogenic E. Coli.更多还原