【摘要】 目的 基于Notch信号通路探讨柴胡皂苷a对肝郁脾虚型抑郁症大鼠模型的影响变化及内在的神经保护调节机制。方法 建立肝郁脾虚型大鼠抑郁模型，利用HE染色观察海马组织形态学变化，运用Western Blotting和RT-PCR法检测Notch信号通路上大鼠海马Notch1、Jagged1、Hes1的蛋白及基因表达情况。结果 HE染色提示模型组大鼠海马区发生不同程度的神经细胞稀疏、排列松散等病理变化。Western Blotting与RT-PCR提示模型组大鼠Notch信号通路上基因及蛋白表达明显下降（P <0.01），柴胡皂苷a对上述结果变化可表现出一定的逆转作用（P <0.01）。结论 柴胡皂苷a干预治疗后可以改善肝郁脾虚型抑郁症状，其内在机制可能是通过调节Notch信号通路发挥海马神经细胞的保护作用来实现。更多还原

【Abstract】 Objective To investigate the effect of saikosaponin a on the neuroprotective regulatory mechanism of liver depression and spleen deficiency syndrome based on Notch signaling pathway. Methods To establish a depression model in rats with liver depression and spleen deficiency, histological changes of hippocampus were observed by HE staining, and protein and gene expression of Notch1, Jagged1 and Hes1 in rat hippocampus were measured by Western Blotting and RT-PCR. Results HE staining indicated that the hippocampus of the model rats showed different degrees of neuronal sparseness and loose arrangement. The Western Blotting and RT-PCR suggested a significantly decreased gene and protein expression on the Notch signaling pathway in the rat model group(P < 0.01). Saikosaponin a could show a statistically significant reversal of these changes in outcome(P < 0.01). Conclusion Saikosaponin a may improve the depressive symptoms of liver depression and spleen deficiency after intervention, and the underlying mechanism may be the protective role of hippocampus nerve cells by modulating Notch signaling pathway.更多还原