【摘要】 目的 探究多花黄精受根腐病危害后对根际土壤酶活性、细菌群落结构和多样性的影响。方法 以未种植多花黄精(HJCK)、多花黄精健株(HJJ)和病株(HJB)的土壤为材料，测定土壤中过氧化氢酶、蔗糖酶等5种酶活性，采用Illumina MiSeq高通量测序技术对土壤样品进行土壤细菌多样性、丰度并聚类分析。结果 多花黄精根腐病的发生对土壤中过氧化氢酶、蔗糖酶、脲酶、酸性磷酸酶和酸性蛋白酶的活性有显著影响；所有土壤样品中优势菌门为变形菌门Proteobacteria、酸杆菌门Acidobacteria、绿弯菌门Chloroflexi、拟杆菌门Bacteroidetes、放线菌门Actinobacteria、芽单胞菌门Gemmatimonadetes，优势菌属为uncultured＿bacterium＿c＿Subgroup＿6、uncultured＿bacterium＿f＿ Gemmatimonadaceae、uncultured＿ bacterium＿f＿Microscillaceae、MND1、硝化螺旋菌属Nitrospira和鞘氨醇单胞菌属Sphingomonas。三组土壤细菌多样性差异不大，但丰度差异显著。以HJCK中细菌丰度最高，其次为HJB。三组土壤中细菌丰度聚类结果也可明显的将三个样品归为三类。结论 多花黄精感染根腐病后，根际土壤中酶活性明显降低，且细菌相对丰度减少。更多还原

【Abstract】 Objective To explore the effects of Polygonatum cyrtonema Hua on rhizosphere soil enzyme activity, bacterial communities structure and diversity. Methods The activities of catalase, sucrase, urease, acid phosphatase and acid protease in soil without planting(HJCK), healthy plant(HJJ) and diseased plant(HJB) were analyzed. The diversity, abundance and cluster analysis of the soil bacteria were carried out after high-throughput sequencing by Illumina Next-generation sequencing platform. Results The occurrence of root rot disease had significant effects on the activities of catalase, sucrase, urease, acid phosphatase and acid protease activity. The phyla included Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, and Bacteroidetes, while the genera consisted of uncultured＿bacterium＿c＿Subgroup＿6、uncultured＿bacterium＿f＿ Gemmatimonadaceae、uncultured＿ bacterium＿f＿Microscillaceae、MND1、Nitrospira and Sphingomonas. The abundance of soil bacteria was significant. HJCK had the highest bacterial abundance, followed by HJB. The bacterial abundance clustering results of the three groups of soil could also clearly classify the three samples into three categories. Conclusion After P. polygonum is infected with root rot, the enzyme activity in rhizosphere soil decreased significantly, and the relative abundance of bacteria also decreased.更多还原