【摘要】 目的 比较3种不同品牌的流式微球分析法多重细胞因子检测试剂(A、B、C试剂)检测结果。方法 选取2020年1月至2021年12月在云南省第一人民医院确诊为急性淋巴细胞白血病(ALL)、急性髓系白血病(AML)、慢性髓系白血病(CML)、噬血细胞综合征(HPS)和多发性骨髓瘤(MM)的50例患者(病例组)分别作为ALL组、AML组、CML组、HPS组和MM组，每组10例。另选取同期健康体检者(健康组)12例。采集各组外周血，获取血清标本。采用A、B、C试剂检测健康组血清标本6种细胞因子[白细胞介素(IL)-2、IL-4、IL-6、IL-10、肿瘤坏死因子(TNF)-α、γ干扰素(IFN-γ)]水平，分析检测结果有无可比性。采用筛选出的试剂检测健康组及病例组血清标本14种细胞因子(IL-4、IL-5、IL-6、IL-8、IL-10、IL-12P70、IL-1β、IL-2、IFN-γ、TNF-α、TNF-β、IL-17A、IL-17F、IL-22)水平，评估各试剂是否满足临床需求。结果 A、B、C试剂分别检测健康组血清6种细胞因子的结果显示，A试剂和C试剂对IL-6、IL-10、IFN-γ的检测结果比较，差异均有统计学意义(P<0.05);A试剂和B试剂对IL-4、IL-6、IL-10、IFN-γ的检测结果比较，差异均有统计学意义(P<0.05);C试剂和B试剂对6种细胞因子的检测结果比较，差异均无统计学意义(P>0.05)。A试剂的检测值范围宽，高、低值差异明显，变异系数(CV)较均一。筛选的A试剂对各组14种细胞因子的检测结果显示，ALL组、AML组、CML组IL-6、IL-8检测结果明显高于正常参考值，HPS组IL-10检测结果明显高于正常参考值，差异均有统计学意义(P<0.05)。结论 不同品牌细胞因子检测试剂存在差异，3种细胞因子检测试剂盒在检测相同的标本时，A试剂的检测值范围宽，高、低值差异明显，可满足临床需求；B试剂和C试剂的检测值范围窄，且较集中。更多还原

【Abstract】 Objective To compare the detection results of multiple cytokine detection reagents(reagents A,B and C) of three different brands of flow microsphere analysis.Methods From January 2020 to December 2021,50 patients(case group) diagnosed with acute lymphoblastic leukemia(ALL),acute myeloid leukemia(AML),chronic myeloid leukemia(CML),haemophilic cell syndrome(HPS) and multiple myeloma(MM) in First People′s Hospital of Yunnan Province were selected as ALL group, AML group, CML group, HPS group and MM group, 10 cases in each group.In addition, 12 healthy subjects were selected as healthy group.Peripheral blood of each group was collected and serum samples were obtained.The levels of 6 cytokines [interleukin(IL)-2,IL-4,IL-6,IL-10,tumor necrosis factor-α(TNF-α),γ interferon(IFN-γ)] in serum samples of healthy group were detected by reagent A,B and C,and the results were compared.The selected reagent was used to detect the levels of 14 cytokines(IL-4,IL-5,IL-6,IL-8,IL-10,IL-12 p70,IL-1β,IL-2,IFN-γ,TNF-α,TNF-β,IL-17 A,IL-17 F and IL-22) in serum samples of the healthy group and the case group.Meanwhile, each brand reagent were evaluated if met clinical needs.Results The results of detecting 6 cytokines in the healthy group with reagent A,B and C showed that there were statistically significant differences in the detection results of IL-6,IL-10 and IFN-γ between reagent A and reagent C(P<0.05).There were significant differences in the detection results of IL-4,IL-6,IL-10 and IFN-γ between reagent A and reagent B(P<0.05).There was no significant difference in the detection results of 6 cytokines between C reagent and B reagent(P>0.05).The detection value range of reagent A was wide, and the difference between high and low values was obvious.Moreover, the coefficient of variation(CV) of detection results of reagent A was uniform.The detection results of 14 cytokines in each group by screening reagent A showed that the detection results of IL-6 and IL-8 in ALL group, AML group and CML group were significantly higher than the normal reference values, and the detection results of IL-10 in HPS group were significantly higher than the normal reference values, and the differences were statistically significant(P<0.05).Conclusion There are differences in cytokine detection reagents of different brands.When the three cytokine detection kits detect the same specimen, reagent A has a wide range of detection values, and the difference between high and low values is obvious, which can meet clinical needs.However, the detection ranges of reagent B and reagent C are narrow and concentrated.更多还原