【摘要】 目的 研究蛋白酶抑制剂BMS-345541和MG-132对人脑胶质瘤细胞凋亡及活力的影响。方法将15株人脑胶质瘤细胞平均分为对照组、BMS-345541组和MG-132组,每组5株。对照组进行传代培养,BMS-345541组加入BMS-345541进行传代培养,MG-132组加入MG-132进行传代培养。采用MTT比色法检测细胞活力,采用流式细胞仪检测细胞凋亡率,采用荧光定量PCR法检测GRP78 m RNA相对表达量,采用蛋白质免疫印迹法检测蛋白表达水平。结果 对照组、BMS-345541组、MG-132组细胞数量分别为(730 000±154)、(257 000±256)、(210 000±333)个,BMS-345541组和MG-132组的细胞数量明显少于对照组,且MG-132组的细胞数量明显少于BMS-345541组,差异有统计学意义(P<0.05);BMS-345541组和MG-132组细胞活力明显低于对照组,细胞凋亡率明显高于对照组,MG-132组的细胞活力高于BMS-345541组,细胞凋亡率低于BMS-345541组,差异有统计学意义(P<0.05);对照组、BMS-345541组、MG-132组的细胞GRP78 m RNA表达水平分别为(2.21±0.08)%、(15.98±1.58)%、(13.55±1.12)%,BMS-345541组和MG-132组的细胞GRP78 m RNA表达水平明显高于对照组,差异有统计学意义(P<0.05);BMS-345541组和MG-132组GRP78、JNK1蛋白表达水平明显高于对照组,差异有统计学意义(P<0.05)。结论 蛋白酶抑制剂BMS-345541和MG-132对人脑胶质瘤细胞的凋亡有诱导作用,可增强患者疗效。更多还原

【Abstract】 Objective To investigate the effects of protease inhibitors BMS-345541 and MG-132 on cell apoptosis and cell viability of human glioma.Methods A total of 15 strains of human glioma cells were divided into control group,BMS-345541 group and MG-132 group,5 strains in each group.The control group was subcultured,BMS-345541 group was added with BMS-345541 and subcultured,MG-132 group was added with MG-132 and subcultured.MTT colorimetric assay was used to detect cell viability,cell apoptosis rate,fluorescence quantitative assay was used to detect GRP78 m RNA expression,western blotting was used to detect protein expression.Results The number of cells in the control group,BMS-345541 group and MG-132 group were(730 000±154),(257 000±256) and(210 000±333) respectively,the number of cells in BMS-345541 group and MG-132 group were significantly lower than that in control group,the number of cells in MG-132 group was significantly lower than that in BMS-345541 group(P<0.05).The cell viability of BMS-345541 group and MG-132 group were significantly lower than that of control group,but the cell apoptosis rate of MG-132 group were lower than that of control group(P <0.05).The cell viability in MG-132 group was higher than that in BMS-345541 group,the cell apoptosis rate in MG-132 group was lower than that in BMS-345541 group(P<0.05).The expression of GRP78 m RNA in control group,BMS-345541 group and MG-132 group were(2.21±0.08)%,(15.98±1.58)%,(13.55±1.12)%.The expression of GRP78 m RNA in BMS-345541 group and MG-132 group were significantly higher than that in control group(P<0.05).The relative expressions of GRP78 and JNK1 protein in BMS-345541 group and MG-132 group were significantly higher than those in control group(P<0.05).Conclusion The protease inhibitors BMS-345541 and MG-132 could induce the cell apoptosis of human glioma and enhance patients’ efficacy.更多还原