【摘要】 目的 体外观察尿毒症血清及枸橼酸钠干预对Th17/Treg平衡的影响，并检测氧化应激指标超氧化物歧化酶(SOD)、丙二醛(MDA)水平，探讨Th17/Treg失衡与氧化应激的关系。方法 选择12只体质量150～180 g的雌性SD大鼠为淋巴细胞来源，提取大鼠脾淋巴细胞。将大鼠分入3组，分别为10%非尿毒症血清组(A组)、10%尿毒症血清组(B组)、10%尿毒症血清+枸橼酸钠(0.5 mg/ml)组(C组)。A组细胞悬液所用培养基由90%RPMI1640培养基成品和10%非尿毒症血清组成；B组细胞悬液所用培养基由90%RPMI1640培养基成品和10%尿毒症血清组成；C组细胞悬液所用培养基由90%RPMI1640培养基成品和10%尿毒症血清组成，同时加入枸橼酸钠，使细胞悬液中枸橼酸钠浓度为0.5 mg/ml。比较3组的Th17细胞比例、Treg细胞比例、Th17/Treg,以及SOD、MDA水平。结果 A组与B组Th17细胞比例比较，差异无统计学意义(P>0.05);C组Th17细胞比例低于B组(P<0.05)。B组Treg细胞比例低于A组(P<0.05);C组Treg细胞比例低于B组(P<0.05)。B组Th17/Treg高于A组(P<0.05);B组与C组Th17/Treg比较，差异无统计学意义(P>0.05)。与A组比较，B组SOD水平降低、MDA水平升高，差异有统计学意义(P<0.05);与B组比较，C组SOD水平升高、MDA水平降低，差异有统计学意义(P<0.05)。结论 尿毒症血清会导致大鼠Th17/Treg失衡和细胞氧化应激，氧化应激是尿毒症血清导致Th17/Treg失衡的机制之一，枸橼酸钠对尿毒症血清导致的氧化应激具有改善作用。更多还原

【Abstract】 Objective To investigate the effects of sodium citrate on Th17/Treg imbalance and oxidative stress in rats induced by uremia serum.Methods Twelve female SD rats with body weight of 150～180 g were selected as the source of lymphocytes, and spleen lymphocytes of rats were extracted.The rats were divided into three groups: 10% non-uremic serum group(group A),10% uremic serum group(group B),and 10% uremic serum+sodium citrate(0.5 mg/ml)group(group C).The medium for cell suspension in group A was composed of 90% finished RPMI1640 medium and 10% non-uremic serum.The medium used for cell suspension in group B was composed of 90% RPMI1640 medium and 10% uremia serum.The medium used for cell suspension in group C was composed of 90% RPMI1640 finished medium and 10% uremia serum, and sodium citrate was added to make the concentration of sodium citrate in cell suspension 0.5 mg/ml.The proportion of Th17 cells, proportion of Treg cells, Th17/Treg, and superoxide dismutase(SOD)and malonaldehyde(MDA)levels of the three groups were compared.Results There was no significant difference in the proportion of Th17 cells between group A and group B(P>0.05).The proportion of Th17 cells in group C was lower than that in group B(P<0.05).The proportion of Treg cells in group B was lower than that in group A(P<0.05).The proportion of Treg cells in group C was lower than that in group B(P<0.05).Th17/Treg in group B was higher than that in group A(P<0.05).There was no significant difference in Th17/Treg between group B and group C(P>0.05).Compared with group A,SOD level in group B was decreased and MDA level was increased, and the differences were statistically significant(P<0.05).Compared with group B,SOD level in group C was increased and MDA level was decreased, and the differences were statistically significant(P<0.05).Conclusion Uremic serum can cause Th17/Treg imbalance and cellular oxidative stress in rats.Oxidative stress is one of the mechanisms that uremic serum can cause Th17/Treg imbalance.Sodium citrate can improve the oxidative stress caused by uremic serum.更多还原