【摘要】 为提供用于稻瘟病生物防治的菌种资源，以稻瘟病菌(Magnaporthe oryzae) Guy11为测试菌，通过平板对峙实验筛选获得了1株对稻瘟病菌具有较强拮抗作用的菌株HKD-6。结合形态学观察、生理生化检测及16S rDNA、gyrA与gyrB基因序列分析，将菌株HKD-6鉴定为贝莱斯芽孢杆菌(Bacillus velezensis)。HKD-6菌株对防治稻瘟病的常用化学杀菌剂稻瘟灵和三环唑具有良好的相容性，且能耐受较高浓度的盐和较宽范围的pH值。该菌株还能够分泌产生蛋白酶和葡聚糖酶，并含有srfAB、ituC、fenD、bamC、bacA和mycB等多种脂肽类抗生素合成相关基因，因此具有防治稻田稻瘟病的潜力。更多还原

【Abstract】 In order to provide microbial resources for biocontrol of rice blast, a strain HKD-6 was proved to effectively inhibit M. oryzae Guy11 by antagonistic experiments. The strain HKD-6 was identified as Bacillus velezensis by morphological observation, physiological and biochemical tests and genetic analysis of 16S rDNA, gyrA and gyrB. The strain HKD-6 shows good compatibility with the commonly used chemical fungicides(Taifengling and Tricyclazole), good salt and pH tolerance.Moreover, the strain HKD-6 not only secretes several extracellular enzymes, such as protease and glucanase, but also harbors many antibiotic-related genes, including srfAB, ituC, fenD, bamC, bacA and mycB. The strain HKD-6 has great potential for biocontrol of rice blast.更多还原