【摘要】 为了进一步降解水稻秸秆青贮饲料中的纤维，提高青贮饲料品质，从湖羊瘤胃液中筛选产阿魏酸酯酶的乳酸菌，鉴定菌株特性，并将其接种到水稻秸秆青贮中，研究其对青贮水稻秸秆纤维降解率和化学成分的影响。结果表明，从湖羊瘤胃液中共筛选出2株高产阿魏酸酯酶的乳酸菌，菌株SR1与鼠李糖乳杆菌的同源性最高，SR2与香肠乳杆菌的同源性最高；SR2的产酸速度比SR1快，培养24 h后培养液的pH值为3.83;2株乳酸菌在pH值4.0～7.0、温度25～37℃、3.0%NaCl条件下的生长状况良好，都能利用多种糖源物质。与CK相比，接种菌株SR2组的水稻秸秆青贮饲料中性洗涤纤维、酸性洗涤纤维和纤维素含量均显著降低(P<0.05),而接种菌株SR1对水稻秸秆青贮饲料纤维含量的影响不大；接种菌株SR2组的水稻秸秆青贮饲料pH值、铵态氮含量最低，乳酸含量最高。综上，香肠乳杆菌SR2可作为水稻秸秆青贮的备选菌株。更多还原

【Abstract】 In order to further degrade the fiber in rice straw silage and improve the quality of silage, ferulic acid esterase-producing lactic acid bacteria were isolated from rumen fluid of Hu sheep, and their characteristics were identified. The strains were inoculated into rice straw silage to study their effects on fiber degradation rate and chemical composition of rice straw silage. The results showed that two strains of lactic acid bacteria with high ferulic acid esterase were isolated from rumen fluid of Hu sheep. Strain SR1 had the highest homology with Lactobacillus rhamnosus, and SR2 had the highest homology with Lactobacillus farciminis. The acid production rate of strain SR2 was faster than that of strain SR1, and the final pH value was 3.83. The two strains of lactic acid bacteria grew well under the conditions of pH 4.0-7.0, temperature 25-37 ℃ and 3.0% NaCl, and they could use various sugar sources. Compared with CK, the contents of neutral detergent fiber, acid detergent fiber and cellulose in the inoculated strain SR2 group decreased significantly(P<0.05), but the inoculated strain SR1 had little effect on the fiber content of rice straw silage. The pH value and ammonium nitrogen content of rice straw silage in the inoculated strain SR2 group were the lowest, and the lactic acid content was the highest. In conclusion, Lactobacillus farciminis SR2 can be used as an alternative strain for rice straw silage.更多还原