【摘要】 金银花是一种重要的大宗中药材，具有多种生物学活性，临床应用广泛。金银花主要以其干燥花蕾入药，花蕾产量和品质与药材质量和经济效益密切相关。该研究利用高效液相色谱-串联质谱（HPLC-MS/MS）方法检测了金银花花发育过程中3种内源激素IAA、ABA和GA₃的含量变化规律。结果表明3种内源激素的变化趋势并不相同，IAA质量浓度呈现出先升高后降低并持平的变化趋势，其含量的持续增加促进了花的发育；ABA和GA₃的质量浓度在白蕾期到金花期的过程中迅速上升，说明高质量浓度的ABA和GA₃促进开花过程。3种激素的平衡在不同发育阶段呈现出不同变化趋势，IAA/ABA比值的升高有利于花蕾初期的发育，而其比值的下降则促进花蕾后期的发育和开花过程；IAA/GA₃和ABA/GA₃比值的升高促进花蕾的发育和开花，而其比值的下降可能促进了花色由白转黄和衰老过程。该研究对揭示植物内源激素调控金银花花发育的机制提供了重要的基础数据。更多还原

【Abstract】 Lonicera japonica Thunb. is an important medicinal plant with a variety of biological activities and is widely used in clinical medicine. L. japonica is mainly used as medicine with its dried flower buds, and the yield and quality of flower buds are closely related to the quality of medicinal materials and economic benefits. In this study, high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was used to detect the changes in the content of three endogenous hormones IAA, ABA and GA₃ during the development of L. japonica flowers. The results showed that the changing trends of the three endogenous hormones were not the same. The concentration of IAA showed a trend of increasing at first, then decreasing and remaining flat, and the continuous increase of its content promoted flower development. The concentrations of ABA and GA₃ increased rapidly from the white bud stage to the golden flowering stage, indicating that high concentrations of ABA and GA₃ promoted the flowering process. The balance of the three hormones showed different trends at different developmental stages. An increase in the IAA/ABA ratio favored the development of buds at the early stage, while a decrease in its ratio promoted the development of buds at the later stage and the flowering process; an increase in the IAA/GA₃ and ABA/GA₃ ratios promoted the development of buds and flowering, while a decrease in their ratios may have promoted the process of flower color changing from white to yellow and senescence. This study provides important basic data for revealing the mechanism of plant endogenous hormones regulating L. japonica flower development.更多还原