节点文献

miR-141-3p对卵巢癌的作用及其机制

Effect of miR-141 on ovarian cancer and its mechanism

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 王秋宇姜平郭哲付小玲李和丽

【Author】 Wang Qiuyu;Jiang Ping;Guo Zhe;Fu Xiaoling;Li Heli;Department of Gynecology, Nanyang Central Hospital;

【机构】 南阳市中心医院妇科

【摘要】 目的:探究miR-141-3p在卵巢癌中的作用及其相关的分子机制。方法:实时荧光定量PCR检测30例卵巢囊肿和30例卵巢癌组织中miR-141-3p和表皮生长因子受体(EGFR)的表达水平。将SKOV3细胞分为NC组(无转染的SKOV3细胞),miR-141-3p组(SKOV3细胞转染miR-141-3p),LV-EGFR组(SKOV3细胞转染LVEGFR)及miR-141-3p+LV-EGFR组(SKOV3细胞转染miR-141-3p及LV-EGFR),通过四氮唑蓝盐化合物(MTS)、Transwell法检测各组SKOV3细胞增殖、侵袭和迁移能力。免疫印迹检测SKOV3细胞中p-AKT、AKT、p-STAT3、STAT3、p-ERK、ERK、p-FAK、FAK、Src、p-Src(Tyr416)、MMP-9和c-Jun蛋白表达。双荧光素酶报告系统、实时荧光定量PCR和免疫印迹等检测miR-141-3p与EGFR3’UTR区域的结合情况,以及其对下游通路蛋白的影响。结果:与正常卵巢组相比,卵巢癌患者组织中miR-141-3p表达水平降低,EGFR表达水平升高。与正常卵巢组相比较,Ⅰ-Ⅱ期和Ⅲ-Ⅳ期卵巢癌组织miR-141-3p表达水平降低,EGFR表达水平升高,卵巢癌组织中miR-141-3p和EGFR表达水平呈负相关。与NC组相比,miR-141-3p组SKOV3细胞增殖、侵袭、迁移数量及p-AKT、p-FAK、p-Src、MMP9及EGFR蛋白表达水平均降低。EGFR的3’UTR区域存在miR-141-3p的结合位点,miR-141-3p组SKOV3细胞中EGFR蛋白表达低于NC组,LV-EGFR组SKOV3细胞中EGFR表达高于NC组,miR-141-3p+LV-EGFR组SKOV3细胞中p-AKT、p-FAK、p-Src、MMP-9及EGFR蛋白表达均高于miR-141-3p组。与NC组相比,miR-141-3p组SKOV3细胞抑制率均升高,与miR-141-3p组相比,miR-141-3p+LV-EGFR组SKOV3细胞抑制率均降低。结论:miR-141-3p通过靶向EGFR抑制卵巢癌的增殖、侵袭和迁移,调控SKOV3细胞对顺铂和紫杉醇的敏感性。

【Abstract】 Objective : To investigate the role of miR-141-3 p in ovarian cancer and its related molecular mechanisms. Methods : Real-time fluorescent quantitative PCR was used to detect the expression levels of miR-141-3 p and epidermal growth factor receptor(EGFR) in 30 ovarian cysts and 30 ovarian cancer tissues. The SKOV3 cells were divided into an NC group(SKOV3 cells without transfection), an miR-141-3 p group(SKOV3 cells transfected with miR-141-3 p), an LV-EGFR group(SKOV3 cells transfected with LV-EGFR) and an miR-141-3 p+LV-EGFR group(SKOV3 cells transfected with miR-141-3 p and LV-EGFR). The cell proliferation, invasion and migration ability of SKOV3 cells in each group were detected by MTS and Transwell methods. Western blotting was used to detect the expression of p-AKT, Akt, p-STAT3, STAT3, p-ERK, ERK, p-FAK, FAK, Src, p-Src(Tyr416), MMP-9 and c-Jun protein in SKOV3 cells. The dual luciferase reporter system, real-time PCR and Western blotting were used to detect the binding of miR-141-3 p to the 3’UTR region of EGFR and its influence on downstream pathway proteins. Results : Compared with the normal ovarian group, the expression level of miR-141-3 p in the tissues of ovarian cancer patients decreased, and the expression level of EGFR increased. Compared with the normal ovarian group, the expression level of miR-141-3 p in stage Ⅰ-Ⅱ and Ⅲ-Ⅳ ovarian cancer tissues decreased, and the expression level of EGFR increased, and the expression levels of miR-141-3 p and EGFR in ovarian cancer tissues were negatively correlated. Compared with the NC group, the number of SKOV3 cell proliferation, invasion, migration and the protein expression levels of p-AKT, p-FAK, p-Src, MMP9 and EGFR decreased in the miR-141-3 p group. There were miR-141-3 p binding sites in the 3’UTR region of EGFR. The expression of EGFR protein in SKOV3 cells in miR-1413p group was lower than that in the NC group. The expression of EGFR in SKOV3 cells in the LV-EGFR group was higher than that in the NC group, and the protein expressions of p-AKT, p-FAK, p-Src, MMP-9 and EGFR in SKOV3 cells in the mi R-141-3 p+LVEGFR group were higher than those in mi R-141-3 p group. Compared with the NC group, the inhibition rate of SKOV3 cells in the mi R-141-3 p group increased. Compared with the mi R-141-3 p group, the inhibition rate of SKOV3 cells in the mi R-141-3 p+LV-EGFR group was reduced. Conclusion: mi R-141-3 p can inhibit the proliferation, invasion and migration of ovarian cancer by targeting EGFR, and regulate the sensitivity of SKOV3 cells to cisplatin and paclitaxel.

  • 【文献出处】 解剖学杂志 ,Chinese Journal of Anatomy , 编辑部邮箱 ,2022年01期
  • 【分类号】R737.31
  • 【被引频次】2
  • 【下载频次】75
节点文献中: 

本文链接的文献网络图示:

本文的引文网络