【摘要】 目的 探究碳量子点黄芩素复合物对金黄色葡萄球菌的抑制作用。方法 采用水热法制备碳量子点，利用γ-缩水甘油醚氧丙基三甲氧基硅烷(KH560)对碳量子点表面进行修饰，通过表面接枝法将黄芩素接枝在碳量子点表面，制备碳量子点黄芩素复合物，利用UV法和IR法进行表征，通过倍量稀释法测定碳量子点-黄芩素复合物对金黄色葡萄球菌的MIC,通过SDS-PAGE测定复合物对金黄色葡萄球菌菌体内可溶性蛋白含量的影响，探究其抑菌机理。结果 碳量子点黄芩素复合物对金黄色葡萄球菌的MIC为0.25mg/mL。复合物通过减少金黄色葡萄球菌蛋白质合成而起到抑菌作用。结论 碳量子点黄芩素复合物可抑制金黄色葡萄球菌的生长，其抑菌作用机制与抑制菌体内蛋白质合成有关。更多还原

【Abstract】 Objective To observe the antibacterial effect of the carbon quantum dot-baicalein composites on growth of Staphylococcus aureus. Method Carbon quantum dots(CDs) were synthesized by hydrothermal method.The composites of C-KH560-Baicalein was prepared by grafting baicalein with CDs via KH560.Surface group composition of the carbon quantum dot-baicalein composites were detected by UV and FT-IR. Determination of MIC of carbon quantum dots-baicalein composite against Staphylococcus aureus by double dilution method, SDS-PAGE was used to determine the effect of carbon quantum dots-baicalein composites on the protein of Staphylococcus aureus.Result The minimum inhibitory concentration of the carbon quantum dots-baicalein composites against Staphylococcus aureus was 0.25 mg/mL. the protein biosynthesis of S. aureus was inhibited,which was further proven by SDS-PAGE.Conclusion The carbon quantum dots-baicalein composites could obviously inhibit the growth of Staphylococcus aureus in vitro. Its main mechanism might be related to the inhibition of protein synthesis.更多还原