【摘要】 目的 明确急诊腹腔镜下胃肠手术使用的无菌手套细菌污染与穿孔情况，为减少医患交叉感染提供依据。方法 选取于我院手术室行急诊腹腔镜下胃肠手术使用的无菌手套1 280只为研究对象，统计手套的细菌污染与穿孔情况，并进行统计学比较。结果 急诊腹腔镜下胃肠手术参与者使用的1 280只无菌手套中，有117只(9.14%)手套发生了穿孔，检出了126株病原菌，最常见的3种依次为:大肠埃希菌32株(25.40%)、金黄色葡萄球菌23株(18.25%)及铜绿假单胞菌19株(15.08%)。左手使用的无菌手套穿孔率显著高于右手，差异有统计学意义(P <0.05);手术主刀及手术器械护士使用的无菌手套穿孔率显著高于主刀助手使用的无菌手套，差异有统计学意义(P <0.01)。手术时间1～3 h及>3 h的手术使用的无菌手套穿孔率显著高于手术时间<1 h的手术使用的无菌手套，差异有统计学意义(P <0.01)。结论 急诊腹腔镜下胃肠手术使用的无菌手套穿孔率较高，术中常被大肠埃希菌、金黄色葡萄球菌及铜绿假单胞菌等细菌污染，手术主刀及器械护士的无菌手套更容易穿孔，建议手术时间超过1 h即更换无菌手套。更多还原

【Abstract】 Objective To clarify the bacterial contamination and perforation of sterile gloves used in emergency laparoscopic gastrointestinal surgery,so as to provide basis for reducing cross infection between doctors and patients. Methods A total of 1 280 sterile gloves used in emergency laparoscopic gastrointestinal surgery in the operating room of our hospital were selected as the research objects. The bacterial contamination and perforation of gloves were counted and compared statistically. Results Among the 1 280 sterile gloves used by emergency laparoscopic gastrointestinal surgery participants,117( 9. 14%) sterile gloves were perforated and 126 pathogenic bacteria were detected,the most common three were Escherichia coli with 32 strains( 25. 40%),Staphylococcus aureus with 23 strains( 18. 25%) and Pseudomonas aeruginosa with 19 strains( 15. 08%). The perforation rate of sterile gloves used by the left hand was significantly higher than that of the right hand,and the difference was statistically significant( P < 0. 05); the perforation rates of sterile gloves used by the surgeon in charge and the surgical instrument nurse were significantly higher than that of the assistant of the surgeon in charge,and the differences were statistically significant( P < 0. 01). The perforation rates of the sterile gloves used in the surgery with the operation time of 1 to 3 hours and > 3 hours were significantly higher than that of the sterile gloves used in the surgery with the operation time of < 1 hour,and the differences were statistically significant( P < 0. 01). Conclusion The sterile gloves used in emergency laparoscopic gastrointestinal surgery have a high perforation rate,they are often contaminated by bacteria such as Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa during the operation. The sterile gloves of the surgeon in charge and the surgical instrument nurse are more likely to be perforated. It is recommended to replace the sterile gloves when the operation time exceeds 1 hour.更多还原