【摘要】 目的 探讨妊娠期糖尿病(gestational diabetes mellitus, GDM)孕妇体内丙酮醛(methylglyoxal, MGO)的表达水平及对孕妇围产期认知功能的影响。方法 收集2019年3月～2020年10月哈尔滨医科大学附属第一医院诊断并分娩的孕妇160例，分为2组：GDM组(n=80)、健康孕妇组(NP组，n=80)。收集50例志愿者为对照组。采集静脉血测量糖化血红蛋白(glycosylated hemoglobin, HbA1c)和血糖水平。应用酶联免疫吸附试验(enzyme-linked immunosorbert assay, ELISA)方法检测MGO、C反应蛋白(C-reactive protein, CRP)、白细胞介素6(interleukin-6,IL-6)、8-异前列腺素F2α(8-iso-prostaglandin F2α,8-iso-PGF2α)、硝基酪氨酸和脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)的表达水平。结果 GDM组中MGO、CRP、IL-6、8-iso-PGF2α、硝基酪氨酸的表达水平明显高于NP组和CG组(P<0.05)。GDM组BDNF表达水平明显低于NP组和CG组(P<0.05)。结论 GDM孕妇存在围产期认知功能减退。更多还原

【Abstract】 Objective To investigate the expression level of methylglyoxal(MGO) in pregnant women with gestational diabetes mellitus(GDM) and the effect on perinatal cognitive function. Methods One hundred and sixty pregnant women diagnosed and delivered in the First Affiliated Hospital of Harbin Medical University from March 2019 to October 2020 were collected and divided into 2 groups: gestational diabetes mellitus(GDM) group(n=80), and healthy pregnant(NP) women group(n=80). Fifty volunteers were collected as control group. Venous blood were collected to measure glycosylated hemoglobin(HbA1 c) and blood glucose. The levels of methylglyoxal(MGO), C-reactive protein(CRP), interleukin-6(IL-6), 8-iso-prostaglandin F2α(8-iso-PGF2α), nitrotyrosine and brain-derived neurotrophic factor(BDNF) were detected by enzyme linked immunosorbent assay(ELISA). Results The levels of MGO, CRP, IL-6, 8-iso-PGF2α, nitrotyrosine in GDM group were significantly higher than those in the NP and CG groups(P<0.05). The level of BDNF in GDM group was lower than that in NP and CG groups(P<0.05). Conclusion Pregnant women with GDM have perinatal cognitive decline.更多还原