【摘要】 通过将GR-5 DNAzyme的序列设计进发生杂交链式反应的发夹DNA中，在引发DNA的触发下，发夹DNA H1与H2反应生成具有重复双螺旋结构的纳米线T-H1-H2-H1-H2…。在H1与H2结合部分存在碱基不配对区域，此处将形成GR-5 DNAzyme结构，在铅离子的存在下，该部分GR-5 DNAzyme的底物链被裂解，HCR产物即被裂解成许多DNA双链小分子从而实现信号扩增，以此构建了简便灵敏的荧光生物传感器。该生物传感器可在30min完成对Pb²⁺的检测，线性范围为0.1μM～7μM,检测限为23.2nM。对实际水样进行检测，加标回收率在96.7%～106.6%之间，相对标准偏差均小于2%。更多还原

【Abstract】 A simple and sensitive biosensor was constructed by incorporating GR-5 DNAzyme sequence into hairpin DNA sequence for hybridization chain reaction.Under the trigger of trigger DNA,hairpin DNA H1 reacts with H2 to generate nanowires T-H1-H2-H1-H2...with repetitive double helix structure.There is a base unpaired region in H1 and H2 binding part, where the GR-5 DNAzyme structure is formed.In the presence of lead ions, the substrate chain of this part of GR-5 DNAzyme is cleaved, so that the HCR product are cleaved into many DNA double-stranded small molecules which caused signal amplification, and thus constructed a simple and sensitive fluorescent biosensor.The biosensor could detect Pb²⁺ within 30 min with a linear range of 0.1μM to 7μM and a detection limit of 23.2 nM.This biosensor was used for Pb²⁺ detection in actual water samples and the recoveries ranged from 96.7%to 106.6%with relative standard deviations less than 2%.更多还原