【摘要】 本文利用黄曲霉素（AFB₁）与AFB₁适体的特异性识别来测定AFB₁的含量。采用MXene材料中的Ti₃C₂纳米材料掺杂金纳米颗粒作为传感基底，以负载金纳米颗粒的金属有机框架材料Au NPs@UiO-66-NH₂为信号探针，利用杂交作用将标记有Au NPs@UiO-66-NH₂的AFB₁适体链结合到DNA₁上。采用差分脉冲伏安法（DPV）,在Tris-HCl缓冲溶液中测定Au NPs@UiO-66-NH₂复合材料的DPV响应电流，构建了signal-off型的电化学适体传感器。传感器对AFB₁的线性响应范围为0.1～110 ng·mL^-1,检出限为0.0331 ng·mL^-1,线性方程为△I=0.5487x+0.9530,实现了对目标物的定量检测。更多还原

【Abstract】 In this paper, the specific recognition of aflatoxin（AFB₁）and AFB₁ aptamer was used to determine the concentration of AFB₁.Au NPs@UiO-66-NH₂,a metal-organic framework loaded with Au NPs, was used as a signal probe.The AFB₁ aptamer chain labeled with Au NPs@UiO-66-NH₂ was bound to DNA₁ through hybridization.Differential pulse voltammetry（DPV）was used to measure the DPV response current of Au NPs@UiO-66-NH₂ composites in Tris-HCl buffer solution, and a signal-off electrochemical aptasensor was constructed.The linear^*response range of the sensor to AFB₁ is 0.1～110 ng·mL^-1,the detection limit is 0.0331 ng·mL^-1,and the linear equation is △I=0.5487x+0.9530,which realizes the quantitative detection of the target.This work provides a new method for the rapid detection of AFB₁.更多还原