【摘要】 本文以肉桂酸甲酯、水合肼及水杨醛为原料，设计合成了一种“turn-on”型离子选择性荧光探针，采用NMR、IR及HRMS对其结构进行了表征，结果表明该探针为N’-[（2-羟苯基）亚甲基]-3-{2-[（2-羟苯基）亚甲基]肼-1-基}-3-苯丙酰肼（ZL）。基于其存在的酰基和邻羟苯基亚甲基胺结构，研究了其对不同金属离子的识别作用，结果Zn²⁺、Mg²⁺和Cd²⁺对探针ZL荧光表现出“turn-on”效应，其荧光分别增强了47、21、24倍，而其他金属离子对其荧光光谱及强度无影响，化合物ZL表现出对Zn²⁺、Mg²⁺和Cd²⁺的高选择性识别和高灵敏检测，其检出限分别为5.5nmol·L^-1、8.4nmol·L^-1、9.9 nmol·L^-1。通过Job’s plot实验表明ZL与Zn²⁺、Mg²⁺和Cd²⁺的结合比为1∶1,结合核磁滴定及Gaussian计算结果，可推测Zn²⁺和Cd²⁺与ZL的酚羟基结合；Mg²⁺与ZL中的酚羟基、羰基氧及席夫碱的氮原子结合。本文设计的荧光探针ZL可望实现对Zn²⁺、Mg²⁺、Cd²⁺的快速高灵敏检测，在生物以及环境样本的监测中具有较好的应用前景。更多还原

【Abstract】 A“turn on”fluorescent probe ZL was designed and synthesized from methyl cinnamate, hydrazine hydrate and salicylaldehyde and characterized by NMR,IR and HRMS.The results showed that the probe was N′-[（2-hydroxyphenyl）methylene]-3-{2-[（2-hydroxyphenyl）methylene]hydrazine-1-yl}-3-phenylpropionyl hydrazine（ZL）.Its recognition of different metal ions was studied because acyl and o-hydroxyphenylmethylene amine structures, The probe ZL exhibited turn-on fluorescence behavior toward Zn²⁺,Mg²⁺ and Cd²⁺and was demonstrated 47,21,24 fold enhancement respectively while other metal ions had no effect on the fluorescence spectrum and intensity.Probe ZL exhibited high selective recognition and sensitive detection for Zn²⁺,Mg²⁺ and Cd²⁺,and its limits of detection（LOD）were 5.5 nmol·L^-1,8.4 nmol·L^-1 and 9.9 nmol·L^-1,respectively.The job’s plot experiment showed that the binding ratio of ZL to Zn²⁺,Mg²⁺ and Cd²⁺were 1∶1,amd can be inferred that Zn²⁺ and Cd²⁺ bind to the phenolic hydroxyl group and Mg²⁺ binds to phenol hydroxyl, carbonyl oxygen and nitrogen atom of Schiff base in ZL from NMR titration and Gaussian calculation.The probe ZL in this paper is expected to realize the rapid and sensitive detection of Zn²⁺,Mg²⁺ and Cd²⁺,and has a good application prospect in the monitoring of biological and environmental samples更多还原