【摘要】 纳米载药脂质体的物理化学稳定性是其实际应用中的关键问题。本文采用薄膜旋转蒸发法-超声法制备了白藜芦醇纳米脂质体（RES-Lip）,通过透射电镜观察其微观形貌，并考察RES-Lip的物理化学稳定性。通过电导法测定了RES-Lip的相变温度（T_m）及其在凝聚过程的凝聚速率常数(K_co)和表观活化能(E_co);采用动态透析-紫外分光光度法研究了温度和pH对RES-Lip降解的影响。结果表明，RES-Lip为球形囊泡结构，粒径小于100nm, RES-Lip的相变温度为64℃,凝聚速率常数K_co随体系温度升高而升高，表观活化能E_co为86.056kJ/mol, RES-Lip的降解符合一级动力学模型，降解的表观活化能E_a为59.3157kJ/mol,降解过程是吸热、自发、熵增过程。本实验制备得到的RES-Lip在4℃、pH 7.40的条件下储存稳定性较好。更多还原

【Abstract】 The physicochemical stability is an important problem of nano-drug-loading liposomes in practical application. In this paper, nano-resveratrol liposomes（RES-Lip） were prepared by film rotary evaporation and ultrasonic method, characterized by transmission electron microscope（TEM）, and the physicochemical stability was investigated. The phase transition temperature（T_m）, condensation rate constant(K_co) and apparent activation energy(E_co) in the condensation process of RES-LIP were measured by the conductivity method. The effects of temperature and pH on the degradation of RES-Lip were studied by dynamic dialysis-ultraviolet spectrophotometer. The results showed that RES-Lip were spherical vesicles with the particle sizes less than 100 nm. The phase transition temperature of RES-Lip was 64 ℃. The condensation rate constant K_co of RES-Lip increased with the increase of temperature. The apparent activation energy E_co was 86.056 kJ/mol. The degradation of RES-Lip belonged to the first-order kinetic model, the apparent activation energy of degradation（E_a） was 59.3157 kJ/mol, and the degradation process was endothermic, spontaneous and entropy-increased process. The RES-Lip obtained in this experiment could be stored at 4 ℃ and pH 7.40 in practical application.更多还原