【摘要】 大规模的钼矿开采造成了严重的环境污染风险，而微生物对节能高效缓解重金属污染问题具有重要意义。立足环境钼污染的生物修复，通过土壤微生物的富集和筛选得到功能菌株并进行16S rRNA鉴定，分析了筛选菌株对MoO₄^2-的作用效能。实验结果表明筛选得到的菌株为黏质沙雷氏菌（Serratia marcescens A2），其在Luria-Bertani培养基（LB）和低磷酸盐培养基（LPM）中对MoO₄^2-分别表现出去除及还原效能。25℃，pH=7.0时Serratia marcescens A2对MoO₄^2-的去除率最高达到了38.55%，钼在菌株体内的分布及FTIR分析结果表明细胞壁在MoO₄^2-去除过程中发挥着重要作用（占钼去除总量的92.14%）。此外，低温会促进菌株对高浓度MoO₄^2-的去除，而偏酸或偏碱性条件会对菌株的去除及还原效能均产生不利影响。上述结果为钼污染的原位生物修复提供了理论依据。更多还原

【Abstract】 The amount mining of molybdenum（Mo） has posed significant environmental risks, and microorganisms are of potential for the mitigation of heavy metal pollution energy-savingly and efficiently. Based on the bioremediation of Mo pollution, the functional strains were obtained by enrichment and isolation of soil microorganisms and identified by 16S rRNA. The efficacy of the obtained strain on MoO₄^2- was also analyzed. It was confirmed that the isolated strain was Serratia marcescens A2, which can remove and reduce MoO₄^2- in Luria-Bertani medium（LB） and low phosphate medium（LPM）,respectively. The removal rate of MoO₄^2- reached 38.55% at 25 ℃ and pH 7.0. The distribution of Mo in Serratia marcescens A2 and the results of FTIR showed that the cell wall performed an essential role in the MoO₄^2- -removal process(92.14% of the total MoO₄^2- removed). In addition, a non-neutral environment could seriously inhibit the removal and reduction of MoO₄^2- by the strain, while the low temperature could promote the removal of MoO₄^2- at high concentrations. This study provides a theoretical basis for the in situ microbial remediation of Mo-contaminated environments.更多还原