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气相色谱–质谱法测定速溶咖啡中糠醛和5-羟甲基糠醛
Determination of furfural and 5-hydroxymethylfurfural in instant coffee by GC-MS method
【摘要】 建立测定速溶咖啡中糠醛和5-羟甲基糠醛的气相色谱–质谱法。样品经乙腈提取,100 mg C18、50 mg强阳离子交换剂(SCX)、150 mg无水MgSO4净化,经HP-INNOWAX毛细管柱分离,选择离子扫描(SIM)模式,同位素内标法定量。糠醛、5-羟甲基糠醛的质量浓度在0.001~8.0μg/mL范围内线性关系良好,相关系数大于0.999,方法检出限为0.080~0.083 mg/kg,平均回收率为91.8%~96.8%,相对标准偏差为2.8%~6.5%(n=5)。该方法简单快速、灵敏可靠,为速溶咖啡中糠醛、5-羟甲基糠醛的提取和检测提供了可靠的技术支撑。
【Abstract】 A gas chromatography-mass spectrometry(GC-MS) method for the determination of furfural and 5-hydroxymethylfurfural in instant coffee was established. The samples were extracted with acetonitrile, purified with 100 mg C18, 50 mg strong cation exchange(SCX) and 150 mg anhydrous MgSO4, separated by HP-INNOWAX capillary column,selected ion scanning(SIM) mode and quantified by isotope internal standard method. Furfural and 5-hydroxymethylfurfural showed a linear relationship in the range of 0.001-8.0 μg/mL with the correlation coefficient more than 0.999, the detection limit of the method was 0.080-0.083 mg/kg, the average recovery was 91.8%-96.8%, and the relative standard deviation was 2.8%-6.5%(n=5). The method is simple, rapid, sensitive and reliable, which provides a reliable technical support for the extraction and detection of furfural and 5-hydroxymethyl furfural in instant coffee.
【Key words】 gas chromatography-mass spectrometry; instant coffee; furfural; 5-hydroxymethylfurfural;
- 【文献出处】 化学分析计量 ,Chemical Analysis and Meterage , 编辑部邮箱 ,2022年12期
- 【分类号】O657.63;TS273
- 【下载频次】38