【摘要】 目的 研究黄芩苷对缺氧乳腺癌干细胞MDA-MB-231增殖及低氧诱导因子-1α(hypoxia inducible factor-1, HIF-1α)、胰岛素样生长因子-Ⅱ（insulin-like growth factor, IGF-Ⅱ）、L-选择素（L-selectin）的影响。方法 四甲基偶氮唑蓝法检测不同浓度（12.5、25.0、50.0、100.0、200.0 mg/L）黄芩苷对缺氧乳腺癌干细胞MDA-MB-231的细胞生长抑制率的影响，筛选出合适的黄芩苷浓度。将缺氧乳腺癌干细胞MDA-MB-231随机分为10组：对照组、BL组（黄芩苷低浓度50.0 mg/L）、BM组（黄芩苷中浓度100.0 mg/L）、BH组（黄芩苷高浓度200.0 mg/L）、DDP组（顺铂）、M组（2-甲氧雌二醇）、BL+M组、BM+M组、BH+M组、DDP+M组。RT-PCR法检测HIF-1α、IGF-Ⅱ、L-selectin mRNA的表达，Western blot检测HIF-1α、IGF-Ⅱ、L-selectin蛋白的表达。结果 与对照组比较，DDP组细胞生长抑制率明显升高（P<0.05），不同浓度黄芩苷的细胞生长抑制率均明显升高（P<0.05）。与黄芩苷200.0 mg/L比较，其他浓度黄芩苷的细胞生长抑制率均明显降低（P<0.05）,DDP组细胞生长抑制率明显升高（P<0.05）。与对照组比较，其余9组组HIF-1α、IGF-Ⅱ、L-selectin蛋白和m RNA表达均明显降低（P<0.05）。与BL组比较，BM组、BH组、BL+M组HIF-1α、IGF-Ⅱ、L-selectin蛋白和mRNA表达均明显降低（P<0.05）。与BM组比较，BM+M组HIF-1α、IGF-Ⅱ、L-selectin蛋白和mRNA表达均明显降低（P<0.05）。与BH组比较，BH+M组HIF-1α、IGF-Ⅱ、L-selectin蛋白和mRNA表达均明显降低（P<0.05）。结论 黄芩苷能抑制缺氧乳腺癌干细胞的增殖，可能通过调节HIF-1α/IGF-Ⅱ信号通路及L-selectin的表达抑制乳腺癌干细胞的增殖。更多还原

【Abstract】 Objective To study the effects of baicalin on proliferation of hypoxic breast cancer cells MDA-MB-231, and on hypoxia-inducible factor-1α(HIF-1α), insulin-like growth factor-Ⅱ(IGF-Ⅱ) and L-selectin expression. Methods MTT assay was employed to detect inhibiting effect of different concentrations of baicalin(12.5, 25.0, 50.0, 100.0, 200.0 mg/L) on growth of hypoxic breast cancer stem cells MDA-MB-231, therefore identif ying the appropriate baicalin concentration. The hypoxic breast cancer stem cells MDA-MB-231 were randomly divided into 10 groups: control group, BL group(baicalin low concentration50.0 mg/L), BM group(baicalin medium concentration 100.0 mg/L), BH group(baicalin high concentration 200.0 mg/L), DDP group(cisplatin), M group(2-methoxyestradiol), BL+M group, BM+M group, BH+M group and DDP+M group. RT-PCR was employed to detect the expression of mRNA in HIF-1α, IGF-Ⅱ and L-selectin. Western blot was applied to detect the protein expression of HIF-1α, IGF-Ⅱ and L-selectin. Results Compared with the control group, the cell growth inhibition rate of DDP group and the cell growth inhibition rates at different concentrations of baicalin significantly increased(P<0.05). Compared with 200.0 mg/L baicalin, the cell growth inhibition rates of other concentrations of baicalin significantly decreased(P <0.05),while the cell growth inhibition rate of DDP group significantly increased(P <0.05). Compared with the control group, the protein and mRNA expression of HIF-1α, IGF-Ⅱ and L-selectin in the other 9 groups significantly decreased(P <0.05).Compared with the BL group, the protein and mRNA expression levels of HIF-1α, IGF-Ⅱ and L-selectin in the BM, BH and BL+M groups significantly decreased(P<0.05). Compared with the BM group, the protein and m RNA expression levels of HIF-1α, IGF-Ⅱ and L-selectin in the BM+M groups significantly decreased(P<0.05). Compared with the BH group, the protein and m RNA expression levels of HIF-1α, IGF-Ⅱ and L-selectin in the BH+M group significantly decreased(P<0.05). Conclusion Baicalin can inhibit the proliferation of hypoxic breast cancer stem cells. It may inhibit proliferation of breast cancer stem cells by regulating HIF-1α/IGF-Ⅱ signaling and L-selectin expression.更多还原