【摘要】 目的：运用DPPH-UPLC-Q-Exactive-MS法研究藤茶的抗氧化活性成分。方法：以不同炮制品的藤茶为对象，考察其清除DPPH自由基的能力，并运用UPLC-Q-Exactive-MS技术对藤茶的抗氧化成分进行定性分析。结果：藤茶提取物对DPPH自由基清除能力强，具有较好的抗氧化活性，UPLC-Q-Exactive-MS分析共鉴定了18个化合物，其中黄酮类化合物15个。结论：应用DPPH抗氧化活性筛选及UPLC-Q-Exactive-MS技术能够快速准确地发现藤茶抗氧化活性成分，本研究结果可为进一步研究藤茶抗氧化的药效物质研究提供参考。更多还原

【Abstract】 Objective: To investigate the antioxidant active components of Ampelopsis megalophylla Diels et Gilg based on DPPH-UPLC-Q-Exactive-MS.Methods: The different processed products of Ampelopsis megalophylla Diels et Gilg were observed for the ability to scavenge free radicals, and the UPLC-Q-Exactive-MS technique was used to perform a qualitative analysis of the antioxidant components of Ampelopsis megalophylla Diels et Gilg.Results: The extract of Ampelopsis megalophylla Diels et Gilg had a strong ability to scavenge free radicals and showed a good antioxidant activity.A total of 18 compounds were identified by UPLC-Q-Exactive-MS,among which there were 15 flavonoids.Conclusion: The DPPH screening method for antioxidant activity and the UPLC-Q-Exactive-MS technique can be used to identify the active antioxidant components of Ampelopsis megalophylla Diels et Gilg, which provides a reference for further research on the material basis for the antioxidant effect of Ampelopsis megalophylla Diels et Gilg.更多还原