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种子萌发期粳稻种质资源耐旱性综合评价

Comprehensive Evaluation of Drought Tolerance of Japonica Rice Germplasm Resources at Seed Germination Stage

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【作者】 张娟伟石亚飞路旭平杨文伟田蕾李培富张振海罗成科

【Author】 ZHANG Juanwei;SHI Yafei;LU Xuping;YANG Wenwei;TIAN Lei;LI Peifu;ZHANG Zhenhai;LUO Chengke;College of Agriculture, Ningxia University;Institute of Crop Science,Ningxia Academy of Agricultural and Forestry Sciences;

【通讯作者】 罗成科;

【机构】 宁夏大学农学院宁夏农林科学院农作物研究所

【摘要】 水资源短缺一直是制约西北干燥区水稻生产的主要因素之一,在水稻某一或全生育期节水有利于缓解该区域农业用水。为挖掘水稻耐旱种质资源和培育耐旱品种,对西北干燥区种植的粳稻种质资源进行种子萌发期的耐旱性鉴定和评价,本研究首先以PEG-6000为渗透剂对6份不同耐旱性水稻材料进行模拟干旱处理,研究了粳稻种子在0%、5%、10%、15%、20%的PEG-6000溶液胁迫下的萌发情况,分析了种子的相对发芽势、相对发芽率、相对根长、相对芽长、活力指数、相对根芽鲜重、相对根芽干重和萌发耐旱指数的变化。结果表明,15%PEG-6000可作为粳稻种子萌发期耐旱性鉴定的最佳浓度。在此基础上,进一步利用该处理浓度对139份不同来源的粳稻种质资源进行萌发期耐旱性鉴定,以筛选浓度时选用的8个耐旱指标作为评价指标,通过主成分分析法确定了相对发芽势、相对芽长、活力指数、萌发耐旱指数为4个关键指标,利用隶属函数结合权重分析法得到了萌发期粳稻种质资源耐旱性的综合评价值D。根据D值排序,筛选鉴定到萌发期耐旱性强的5个粳稻种质:松粳3号、通院515、龙粳807、松粳20和Farry。通过聚类分析将139份粳稻种质资源划分为4个亚群,第Ⅰ和Ⅲ亚群(除个别种质为中等耐旱种质外)均为旱敏感亚群,第Ⅱ亚群为耐旱和中等耐旱混合亚群,第Ⅳ亚群为中等耐旱亚群。本研究构建的聚类图中,活力指数与D值表现出较好的一致性。本研究结果可为种子萌发期粳稻种质资源耐旱性快速鉴定和评价提供参考。

【Abstract】 The shortage of water resources has always been one of the main factors restricting rice production in the Northwest aird area in China. Saving water in a certain or whole growth period of rice is conducive to alleviate agricultural water usage in this area. Therefore, it is of great significance to identify and evaluate drought tolerance of japonica rice germplasm resources in northwest arid area for digging out drought tolerant rice germplasm resources and breeding drought tolerant varieties. In this study, six rice materials with different drought tolerance were firstly treated with PEG-6000 an osmotic agent to simulate drought, then the germination of japonica rice seeds under the stress of 0%, 5%, 10%, 15%, and 20% PEG-6000 solution were investigated via analyzing the relative germination potential, relative germination rate, relative root length, relative shoot length, vigor index, relative root shoot fresh weight, relative root shoot dry weight and germination drought tolerance index. The results displayed that the optimum concentration of PEG-6000 for identifying drought tolerance of japonica rice seeds during germination was 15%. On this basis, 139 japonica germplasm resources with different origins were further evaluated for drought tolerance at the germination stage. 8 drought tolerance indexes selected in the concentration screening were used as evaluation indexes, and the relative germination potential, relative shoot length, vigor index and germination drought tolerance index were identified as 4 key indexes by principal component analysis. The comprehensive evaluation value D of drought tolerance of japonica germplasm resources at germination stage was obtained by using the affiliation function combined with weight analysis. Five japonica germplasm with strong drought tolerance at germination stage were screened and identified according to the ranking of D value: Songjing 3, TongYuan 515, Longjing 807, Songjing 20 and Farry. 139 japonica germplasm resources were classified into four subgroups by cluster analysis, and subgroups Ⅰ and Ⅲ(except for some germplasm with medium drought tolerance) were drought-sensitive subgroups, subgroup Ⅱ was a mixed drought-tolerant and medium drought-tolerant subgroup, and subgroup IV was a medium drought-tolerant subgroup. The vigor index and D value illustrated good agreement in the cluster diagram constructed in this study, which could provide reference for rapid identification and evaluation of drought tolerance of japonica rice germplasm resources during seed germination.

【基金】 宁夏农业育种专项课题(2018NYYZ0301)
  • 【文献出处】 核农学报 ,Journal of Nuclear Agricultural Sciences , 编辑部邮箱 ,2022年11期
  • 【分类号】S511.22
  • 【下载频次】297
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