【摘要】 为开发新型安全的植物源沙糖橘保鲜剂，本研究以沙糖橘为研究对象，探讨丁香、肉桂、鱼腥草和广藿香对沙糖橘的防腐保鲜效果，并以普鲁兰多糖为成膜剂，通过单因素和响应面试验筛选出最佳复合涂膜保鲜剂。结果表明，0.20 mg·mL^-1丁香水提液+0.10 mg·mL^-1鱼腥草水溶液+1.50%普鲁兰多糖复合涂膜保鲜剂处理组对沙糖橘的保鲜效果最佳。与空白对照组CK1和蒸馏水处理组CK2相比，在温度28℃、相对湿度75%、贮藏时间10 d的条件下，复合涂膜保鲜剂组处理的沙糖橘失重率分别降低了10.89、9.04个百分点，腐烂率分别降低了15、30个百分点，呼吸强度分别降低了42.97%、36.62%,可溶性固形物含量分别提高了2.70、3.51个百分点，V_C含量分别提高了57.72%、23.29%,可滴定酸含量分别提高了0.15、0.16个百分点。同时，复合涂膜保鲜剂处理组能诱导沙糖橘中过氧化物酶（POD）和多酚氧化酶（PPO）防御相关酶的活性，显著减少丙二醛含量的积累，从而提高其自身抗病性。复合涂膜保鲜剂组和0.225 g·L^-1咪鲜胺阳性对照组的保鲜效果无显著差异。本研究为开发新型安全的植物源沙糖橘保鲜剂提供了理论支持。更多还原

【Abstract】 In order to develop a new plant-resource Citrusreticulata preservative, the effects of Syzygium aromaticum, Cinnamomum cassia, Houttuynia cordata and Pogostemon cablin on the preservation of Citrusreticulata were studied, and the best preservative was selected by single factor and response surface test. The results showed that the treatment group with 0.20 mg·mL^-1 Syzygium aromaticum water extract + 0.10 mg·mL^-1 Houttuynia cordata aqueous solution +1.50% pullulan polysaccharide compound coating preservative had the best preservation effect on Citrusreticulata. Comparing with CK1（control group） and CK2（coated with sterile water）, the weight loss of the compound coating preservative group was reduced by 10.89 and 9.04 percentage points, respectively, under the condition of 28℃, relative humidity75% and 10 days of storage; The spoilage loss was reduced by 15 and 30 percentage points, respectively. The respiratory intensity was reduced by 42.97% and 36.62%, respectively. The soluble solid content was increased by 2.70 and 3.51 percentage points, respectively. The content of V_C was increased by 57.72% and 23.29%, respectively. Titratable acid content was increased by 0.15 and 0.16 percentage points, respectively. Meanwhile, the compound coating preservative treatment group increased activities of the defense enzymes involved in POD and PPO, significantly reduced the content of malondialdehyde, improving Citrusreticulata’s disease resistance. The fresh-keeping effects of the composite coating treatment had no significant difference from that in positive control group(0.225 g·L^-1 prochloraz). Our data provide theoretical support for the development of new safe preservatives for Citrusreticulata.更多还原