【摘要】 湖南处于茶树遗传演化的过渡带，茶树种质资源丰富，这些资源不仅在植物学特征上差异显著，而且在茶类适制性、适应性、耐寒性等方面各有特点，然而目前对湖南茶树资源功能成分差异的深度解析尚缺乏深入研究。为了进一步探明湖南典型地方茶树种质资源的功能成分差异，本研究采用超高效液相色谱-串联四极杆质谱(UPLC-MS/MS)分析技术，对湖南典型地方茶树种质资源城步峒茶群体(CBDC)、江华苦茶群体(JHKC)、汝城白毛茶群体(RCBM)、安化群体(AHQT)进行代谢物检测。在4个群体中共检测到609个代谢物，主成分分析(PCA)结果表明4个群体的代谢物存在显著差异，安化群体与城步峒茶群体代谢物的相似度较高，与汝城白毛茶群体的代谢物相似度最低；以栽培型安化群体为对照组，基于正交偏最小二乘法判别分析(OPLS-DA)模型，共检测到302个差异代谢物，主要为氨基酸类、酚酸类、黄酮类、鞣质类、生物碱类等。3个群体的共有显著差异代谢物为50个，种类最多的为黄酮类，其次为酚酸类，均以含量增加为主。城步峒茶、江华苦茶和汝城白毛茶群体的特有差异代谢物分别有52、37和66个。本研究除检测到茶叶中部分已有研究报道的差异代谢物，还检测到一些茶叶中报道较少的差异代谢物，这些物质可为后期特异茶树种质资源的挖掘与选育提供重要参考。更多还原

【Abstract】 Hunan province is located in a transitional zone of tea evolution, where tea germplasms resources are abundant. These germplasm have not only significantly different botanical characteristics, but also varied tea suitability, adaptability, cold tolerance, and etc. However, there were insufficient analyses on the component differences of tea resources in Hunan province. In order to further explore the differences in functional component of Hunan local tea germplasm, and provide the basis for mining and utilizing these tea resources. The metabolites of Chenbu Dongcha(CBDC), Jianghua Kucha(JHKC), Rucheng Baimaocha(RCBM), and Anhua population(AHQT) in Hunan province were detected using UPLC-MS/MS(with the cultivated landrace AHQT as the control group) and identified using mass spectrometry database. A total of 609 metabolites were detected in the 4 populations. Principal component analysis(PCA) showed that the metabolites among the 4 populations were significantly different. The metabolites of AHQT population had the highest similarity to that of CBDC population, and the lowest to that of RCBM populations. With AHQT population as the reference, a total of 302 differential metabolites were detected based on the OPLS-DA model, mainly including amino acids, phenolic acids, flavonoids, tannins, and alkaloids. Among which fifty common differential metabolites were detected in the 3 populations; they were mainly flavonoids and phenolic acids. There were 52, 37, and 66 unique differential metabolites that were detected in CBDC, JHKC, and RCBM, respectively. Except for some reported differential metabolism, we also detected some less concerned differential metabolites in tea research. These differential metabolisms will provide an important reference for tea germplasm excavation and breeding.更多还原