【摘要】 在冬季早期典型的边际天气条件下，环境温度较高，人工造雪的效率较低，水资源的消耗较高，使用生物冰核提高人工造雪效率技术已经被广泛采用，但是造价昂贵，不利于在国内大范围推广.通过筛选优化新的菌种，制备冰核蛋白，在不同温度下，添加不同浓度冰核蛋白进行冻融实验.发现添加该种冰核蛋白能够对相变速度产生影响：在-5℃时添加浓度为2.3×10^-4mg/mL或1.15×10^-4mg/mL的冰核蛋白溶液能够有效加快冻结速度，且成冰的透明度较好；添加冰核蛋白明显有助于减缓消融，使冰保持的时间更长.可见，筛选优化的冰核蛋白在初步试验中，能够在较高的环境温度下使水较快冻结且减缓消融速度，有利于降低成本并在未来滑雪场中大规模使用.更多还原

【Abstract】 In the typical marginal weather conditions in early winter,the ambient temperature is high,the artificial snowmaking efficiency is low,and the water resource consumption is high.The technology of using biological ice nucleation to improve the artificial snowmaking efficiency has been widely adopted,but the cost is expensive,which is not conducive to large-scale promotion in China.By screening and optimizing new strains,ice nucleation proteins were prepared.Ice nucleation proteins with different concentrations were added to perform freeze-thaw experiments at different temperatures.It is found that adding this ice nucleation protein can affect the phase transition speed.The ice nucleation protein addition solution with 2.3×10^-4mg/mL or 1.15×10^-4mg/mL at -5℃ can effectively accelerate the freezing speed,and the transparency of ice obtained is better.The addition of ice nucleation protein is obviously helpful to the slow down ablation.In preliminary experiments,the optimized ice nucleation protein in this study can make the water freeze faster and slow down the ablation rate at a higher ambient temperature,and the cost is significantly reduced.It will be beneficial to the large-scale use in ski resorts in the future.更多还原