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厌氧氨氧化菌在UFBAR中培养及脱氮性能研究
Study on Anammox Cultivation and Nitrogen Removal In Upflow Fixed Bed Anaerobic Reactor
【摘要】 采用两种氮源和无机碳源配制不同的人工模拟废水,在升流式固定床厌氧生物反应器(UFBAR)中对厌氧氨氧化菌进行培养与驯化,分别为试验Ⅰ和试验Ⅱ。通过测定进、出水NH4+-N、NO2--N、NO3--N质量浓度及pH值等,对反应器中厌氧氨氧化菌的培养及其脱氮性能进行了表征。结果表明,试验Ⅰ中反应器脱氮效果较差,基本不符合厌氧氨氧化反应特征;试验Ⅱ反应器运行10 d后,NH4+-N与NO2--N最低去除率分别为95.9%、96.2%,TIN去除率维持在80%以上,RsⅡ(ΔNO2--N/ΔNH4+-N)均值约为1.35,在理论值1.32附近,第145 d测定单位填料脱氮速率为7.22 kg-N/(m3·d),反应器运行稳定,厌氧氨氧化菌活性良好。
【Abstract】 Two nitrogen and inorganic carbon sources were used to formulate different artificial simulated wastewaters,and anammox bacteria were cultured and domesticated in an ascending-flow fixed-bed anaerobic bioreactor(UFBAR) for Experiment Ⅰ and Experiment Ⅱ, respectively. The culture of anammox bacteria in the reactor and their denitrification performance were characterized by measuring the influent and effluent NH4+-N, NO2--N, NO3--N and pH. The results show that the nitrogen removal efficiency of the reactor in experiment I is poor, which does not accord with the anaerobic ammonia oxidation reaction characteristic. After 10 days of operation, The minimum removal rates of NH4+-N and NO2--N were95.9% and 96.2%, respectively, TIN removal rate remained above 80%, the mean RsⅡ(ΔNO2--N/ΔNH4+-N) is about 1.35, near the theoretical value of 1.32. In day 145, the nitrogen removal rate per unit packing was measured and the value was about 7.22 kg-N/(m3·d). Exeperiment results showed the stable reactor operation and high Anammox activity.
【Key words】 biological nitrogen removal; anammox; microbial culture; nitrogen removal efficiency;
- 【文献出处】 河南科技 ,Henan Science and Technology , 编辑部邮箱 ,2022年16期
- 【分类号】X703;X172
- 【下载频次】136