【摘要】 【目的】降低养殖废水中有机氮含量。【方法】从精养池塘底泥中分离得到一株具有高效氨化能力的芽孢杆菌,命名为ZXL-7。通过形态学观察、生理生化实验和16S rDNA序列分析进行菌株鉴定;利用单因素试验研究培养基组成对菌株ZXL-7氨化效果的影响。根据单因素试验结果,采用响应面法对影响氨化效果较大的因素进行了优化。【结果】结果表明,高效氨化能力菌株ZXL-7为巨大芽孢杆菌（Bacillus megaterium）。优化的氨化培养基组成为:丁二酸钠28.04 g/L,酵母提取物8.73 g/L,KH₂PO₄ 2.05 g/L,MgSO₄·7H₂O 1.58 g/L,MnSO₄·H₂O 0.61 g/L,NaCl 3.00 g/L。【结论】菌株ZXL-7对降低养殖废水中的有机氮含量具有一定的应用价值。更多还原

【Abstract】 【Objective】It is to reduce the content of organic nitrogen in aquaculture wastewater. 【Method】A Bacillus with high ammoniation ability which was named ZXL-7 was isolated from the sediment of intensive culture pond. Phyziological biochemical experiments. The effect of medium composition on ammoniation of strain ZXL-7 was studied by single-factor test. Based on the single-factor test results, the factors affecting the ammoniation were optimized by response surface method. The results showed that strainZXL-7 was identified as Bacillus megaterium.【Result】The optimized ammoniation medium were: Sodium succinate 28.04 g/L, Yeast extract 8.73 g/L, KH₂PO₄ 2.05 g/L, MgSO₄·7 H₂O 1.58 g/L, MnSO₄·H₂O 0.61 g/L and NaCl 3 g/L.【Conclusion】Strain ZXL-7 has certain application value in reducing the content of organic nitrogen in aquaculture wastewater.更多还原