【摘要】 为了鉴定异性双胎牛性器官发育紊乱疾病——牛自由马丁(freemartinism)综合征，试验选择7头中国荷斯坦异性孪生双胎的母牛进行微滴数字PCR(droplet digital PCR,ddPCR)检测，并根据异性孪生母犊不孕的个体染色体为XX/XY嵌合、正常母牛染色体为XX来判定是否为嵌合体。结果表明：此7个样本均存在XX/XY染色体嵌合，均为异性孪生不孕母犊。说明ddPCR方法用于鉴定牛自由马丁现象是有效的。更多还原

【Abstract】 To identify bovine freemartin syndrome, a disease caused by disorder of sexual organ development in co-twin heterosexual bovines, the peripheral bloods of seven Chinese Holstein freemartin cows were used to detect the existence of the XX/XY chimera by the way of droplet digital PCR(ddPCR). The determination references was that the karyotypes of infertile twin calves and normal cows should be XX/XY chimera and XX, respectively. The results showed that all seven samples were suffering XX/XY chimera and were hetero-twin infertile, and the ddPCR could be an effective way of identifying the bovine freemartinism.更多还原