【摘要】 为建立含有不同陈化年份、不同产地陈皮药材的六君子汤的指纹图谱，进行方法学考察、相似度分析及聚类分析，为“谱-效”关系研究奠定基础。采用HPLC法进行测定，色谱柱采用Venusil XBP C18(L)(5μm,4.6 mm×250 mm);流动相为乙腈(B)-0.1%甲酸水(A)溶液，梯度洗脱，检测波长为300 nm;柱温30℃;体积流量为1.0 m L/min。对所得各样品的指纹图谱进行整体相似度计算，并对其共有色谱峰峰面积进行聚类分析。建立得到15批六君子汤的HPLC指纹图谱及其对照图谱，其相似度大都符合要求，但有所差异。在共有模式图谱中有69个特征峰，其中指认出14个与陈皮相关的特征峰。15批六君子汤样品的相似度在0.753～0.985，通过聚类分析大致可聚为3类。说明不同产地的药材成分含量存在着差异。该方法简便、可靠，可用于六君子汤中陈皮的质量评价，为进一步完善六君子汤的质量标准及“谱-效”关系研究提供了参考。更多还原

【Abstract】 To establish the fingerprint spectrum of Liujunzi Decoction containing Citri Reticulatae Pericarpium medicinal materials of different aging years and different origins,the study conducts methodological investigation,similarity analysis and cluster analysis to lay the foundation for the subsequent “spectrum-effect”relationship research. Then the study adopts HPLC method,and Venusil XBP C18( L)( 5 μm,4. 6 mm × 250 mm) on chromatographic column. The mobile phase is acetonitrile( B)-0. 1% formic acid water( A) solution with gradient elution,and detection wave length is 300 nm; column temperature is 30℃; the volume flow rate is 1. 0 m L/min. The overall similarity of the fingerprints of each sample is calculated,and cluster analysis of its common chromatographic peak area is done. 15 batches of Liujunzi Decoction’s HPLC fingerprint spectrum and their control spectrum are established. The similarities mostly meet the requirements,but there are some differences. There are 69 characteristic peaks in the shared pattern map,among which14 characteristic peaks related to Citri Reticulatae Pericarpium are identified. The similarity of the 15 batches of Liujunzitang samples is between 0. 753 and 0. 985,which can be roughly grouped into 3 categories through cluster analysis. This indicates there are differences in the content of medicinal ingredients from different origins. This method is simple and reliable,can be used for the quality evaluation of Citri Reticulatae Pericarpium in Liujunzi Decoction,and provides reference for further improvement of the quality standard of Liujunzi Decoction and the research on the relationship between “spectrum and effect”.更多还原