【摘要】 陆生植物与多种土壤微生物群落存在密切互作关系，影响植物的生长和发育。植物微生物群落的系统发育结构由生物群落和环境因素相互作用所决定。文章选取具有不同遗传背景的玉米自交系材料昌7-2和B73,在山东和海南种植，对其根系微生物组进行高通量测序并进行群落组成、多样性及随机森林分析。研究结果表明，玉米根系微生物组对栽培环境变化的响应程度大于基因型，而不同基因型玉米根系微生物组对栽培环境变化的响应程度也不同，同时利用随机森林分析发现若干丰度显著受宿主基因型影响的细菌类群。该工作进一步深化了栽培环境和宿主基因型对玉米根系微生物组影响的认识，为研究玉米根系微生物群落的建立提供了理论基础。更多还原

【Abstract】 Terrestrial plants interact closely with a variety of soil microbial communities, which affects the growth and development of plants. The phylogenetic structure of plant microbial community is determined by the interactions of biological community and environmental factors. In this study, maize inbred lines Chang7-2 and B73 with different genetic backgrounds were selected and planted in Shandong and Hainan. Their root microbiota was explored by high throughput sequencing and the bacterial community composition, diversity and random forest analyses were carried out. The results showed that the response of maize root microbiota to the change of cultivation environment was greater than that of genotype, and the response of maize root microbiota of different genotype to the change of cultivation environment was also different. Meanwhile, through random forest analysis, some bacterial groups whose colonization abundance was significantly affected by the host genotype were found. This work further deepened the understanding of the influence of environment and host genotype on maize root microbiota, and provided a theoretical basis for the study of the establishment of maize root microbial community.更多还原