【摘要】 建立了一种基于铅、镉单克隆抗体的胶体金试纸条方法，用于快速检测纺织品中总铅、总镉含量，通过加标实验及对实际试样进行测试，探讨了试纸条的灵敏度、准确性和稳定性。结果表明：制备胶体金试纸条时，铅、镉单克隆抗体较佳用量分别为50,45μL;试纸条对纺织品中总铅及总镉含量的检出限分别达5.0 mg/kg和0.625 mg/kg,仅需3～5 min即可实现对试样的定性或半定量测试；试纸条对铅、镉离子具有良好的选择性，其灵敏度和稳定性均能满足实际试样的测试要求；该方法操作便捷，准确度高，适用于纺织品的现场快速筛检。更多还原

【Abstract】 A colloidal gold test strip method based on the lead and cadmium monoclonal antibodies was established for rapidly determining the total lead and cadmium in textiles. The sensitivity, accuracy and stability of the test strip were evaluated by adding calibration experiments and testing the actual samples. The results showed that the optimal dosage of lead and cadmium monoclonal antibodies was 50 and 45 μL, respectively, for preparing the colloidal gold test strip; the test limit of the total lead and cadmium in textiles reached 5.0 mg/kg and 0.625 mg/kg respectively, and it took only 3 to 5 min to complete qualitative or semi-quantitative test of samples; the test strip had good selectivity for lead and cadmium ions, and its sensitivity and stability can meet the test requirements of actual samples; and the method had the advantages of convenient operation and high accuracy and was suitable for on-site rapid screening of textiles.更多还原