【摘要】 目的 沙利度胺联合顺铂对宫颈癌Hela细胞的抑制激励及对Elk1信号转导分子活性的影响。方法 本实验采用免疫组织化学(IHC)分析、荧光素酶活性检测、RT-PCR和Western Blot对沙利度胺联合顺铂处理后宫颈癌细胞的凋亡和Elk1转录因子的活性，以及Elk1、c-fos和caspase-8基因和蛋白的表达进行了检测和分析。结果 沙利度胺联合顺铂处理后能显著的抑制Elk1转录因子的活性，显著下调Elk1和c-fos基因和蛋白的表达并显著上调caspase-8基因和蛋白的表达(P<0.05)。且沙利度胺联合顺铂的治疗效果要比它们单独使用更加显著(P<0.05)。结论 沙利度联合顺铂具有抑制宫颈癌细胞的增殖和促进细胞凋亡的作用。更多还原

【Abstract】 Objective To investigate the effects of thalidomide combined with cisplatin on Elk1 signal transduction molecule activity in cervical cancer cells-Hela in vitro.Methods Immunohistochemistry(IHC),luciferase activity assay, RT-PCR and Western Blot were used to detect the cell apoptosis and Elk1 transcription factor activity in cervical cancer cells(Hela) that were treated by thalidomide combined with cisplatin, moreover, the expression levels of Elk1,c-fos, caspase-8 genes and protein expression were detected and analyzed.Results After Hela cellse were treated by thalidomide combined with cisplatin, the activity of Elk1 transcription factor and the expression levels of Elk1,c-fos were significantly down-regulated, however, the expression levels of caspase-8 genes were significantly up-regulated(P<0.05).Moreover the effects of thalidomide combined with cisplatin and protein were superior to those by simple application of thalidomide or cisplatin(P<0.05).Conclusion The Elk1 may be a direct target of thalidomide combined with cisplatin, which can not only decrease the expression and activity of ELK1 in Hela cells, but also can down-regulate the expression levels of phosphorylated Elk1 target gene-c-fos and upregulate the expression levels of the receptor pathway gene-caspase-8,which suggests that the combination of thalidomide and cisplatin has the effects of inhibiting the proliferation of Hela cells and promoting cell apoptosis.更多还原