【摘要】 为检测转sm-Ngt1基因株系后代的遗传稳定性，对连续2年种植的15个转sm-Ngt1基因T4代水稻株系进行了阳性鉴定，并以农业部要求的9对引物对转基因标记物进行检测，同时对其农艺性状进行了统计。结果显示，15个转基因株系中，编号1、3、5、7-9、11-15这11个转基因株系中含有sm-Ngt1基因，除编号8株系外，sm-Ngt1基因在其余10个株系中均能稳定遗传；在9对检测标记物引物中5对能扩增出特异性条带，其中3对引物（NOS-F1/NOS-R1、T35S-F1/T35S-R1、NPTF68/NPTR356）在所有转基因水稻株系后代中（T5、T6代）都能扩增出特异性条带，2对引物（35S-F1/35S-R1、HPTF226/HPTF69）检测结果与sm-Ngt1检测结果相同。结果表明编号1、3、5、7、9、11-15的10个转基因株系中含有35S启动的sm-Ngt1基因和HPT基因能稳定遗传，且这些转基因株系生育期、株高、结实率及千粒重与受体品种之间差异不显著。更多还原

【Abstract】 To detect the genetic stability of the offspring of the transgenic with sm-Ngt1 plant lines,15 T4 rice lines transformed with sm-Ngt1 gene were planted,the positive plants were identified,the genetically modified markers were tested with 9 primer pairs required by the ministry of agriculture and the agronomic trait was counted for two consecutive years. The results showed that the smNgt1 was found in 11 genetically modified rice lines numbered 1,3,5,7-9,11-15 in the 15 lines,and in the another 10 lines except numbered 8 the sm-Ngt1 was genetically stable. There were 5 of 9 primer pairs of detecting markers with amplification strips,of which there were 3 primer pairs NOS-F1/NOS-R1,T35S-F1/T35S-R1,NPTF68/NPTR356 with specific bands in all genetically modified offspring(T5 and T6 generations),and the test results for 2 primer pairs(35S-F1/35S-R1 and HPTF226/HPTF69)were the same as for sm-Ngt1. These results demonstrated that the 10 genetically modified rice lines numbered 1,3,5,7,9,11-15 contained the 35S-sm-Ngt1 and HPT were able to maintain stable genetics,and there was no significant difference in growth period,plant height,seeding rate and thousand grain weight between the modified lines with sm-Ngt1 and the receptor.更多还原