【摘要】 [目的]建立粤糖93-159的再生体系，为进行化学诱变选育新品系奠定基础。[方法]以粤糖93-159嫩叶鞘为外植体，探究分别添加2,4-D、6-BA、NAA和活性炭对甘蔗愈伤组织诱导、分化以及组培苗生根的影响。[结果]粤糖93-159愈伤诱导的最佳培养基为MS+2.0 mg·L^-1 2,4-D+30 g·L^-1蔗糖+7 g·L^-1琼脂，诱导率为95.37%;愈伤分化最佳培养基为MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA+30 g·L^-1蔗糖+7 g·L^-1琼脂，分化率为42.22%;生根最佳培养基为1/2MS+3.0 mg·L^-1 NAA+0.5 mg·L^-1活性炭+30 g·L^-1蔗糖。[结论]建立了粤糖93-159的组织培养再生体系，为甘蔗种苗快繁、遗传转化以及诱变等提供参考。更多还原

【Abstract】 [Purpose] The regeneration system for Yuetang 93-159 was established to lay a foundation for breeding a new sugarcane strain. [Method] The callus induction, differentiation and rooting of regenerated seedings from tender leaf sheaths of sugarcane variety Yuetang 93-159 were investigated after adding different concentrations of 2,4-D, 6-BA, NAA and activated carbon in basic medium, respectively. [Result] The best medium for callus induction was MS+2.0 mg·L^-1 2,4-D+30 g·L^-1 sucrose+7 g·L^-1 agar, resulting in a induction rate of 95.37%; the best medium for differentiation was MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA+30 g·L^-1 sucrose+7 g·L^-1 agar, with a differentiation rate of 42.22%; the best medium for rooting was 1/2 MS+3.0 mg·L^-1 NAA+0.5 mg·L^-1 activated carbon+30 g·L^-1 sucrose. [Conclusion] A tissue culture regeneration system of Yuetang 93-159 was established in this study, which laid a foundation for rapid propagation, genetic transformation and mutation of sugarcane seedlings.更多还原