【摘要】 [目的]了解凌云白毫茶群体的遗传多样性及其之间的亲缘关系，为凌云白毫茶树品种的鉴定、遗传改良等提供参考。[方法]采集25个凌云白毫茶群体共150个个体并提取其基因组DNA,利用4个SSR及4个InDel分子标记结合高通量毛细管电泳进行多态性位点检测，统计条带并分析遗传多样性及构建分子指纹图谱。[结果]凌云白毫茶群体经8对引物扩增后共检测出98个等位基因，引物扩增出来的等位基因为7～17个，平均为12.25个；有效等位基因数(Ne)为1.139～3.290,均值为2.077。在群体水平上，凌云白毫茶群体表现出较低的遗传多样性，Ne介于1.048～3.502之间，观测杂合度(Ho)介于0.000～0.271之间，期望杂合度(He)介于0.035～0.573之间，香农信息指数(I)介于0.056～1.181之间。[结论]明确了25个凌云白毫茶群体相互之间的亲缘关系，并构建了各自特异的分子指纹代码，为凌云白毫茶种质资源保护及品种选育提供了依据。更多还原

【Abstract】 [Purpose] A better understanding of the genetic diversity and relationship of Lingyun-baihaocha tea populations will provide a reference for the identification and genetic improvement of Lingyun-baihaocha tea varieties. [Method] Four simple sequence repeats(SSRs) and 4 insertion/deletion(InDel) markers were used to analyze the genetic diversity and construct the molecular fingerprinting of 150 individuals from 25 Lingyun-baihaocha tea populations. [Result] Ninety-eight alleles were detected by 8 pairs of primers. The number of alleles amplified by each primer pair ranged from 7 to 17(average 12.25), with the number of effective alleles(Ne) fluctuating between 1.139 and 3.290(average 2.077). The Lingyun-baihaocha tea plants showed a relatively low genetic diversity at the population level considering the Ne of 1.048-3.502, the observed heterozygosity(Ho) of 0.000-0.271, the expected heterozygosity(He) of 0.035-0.573 and the Shannon′s information index(I) of 0.056-1.181. [Conclusion] Illuminations on the genetic diversity and molecular fingerprinting of 25 Lingyun-baihaocha tea populations provided a theoretical basis for the protection of Lingyun-baihaocha tea germplasm and breeding of elite cultivars.更多还原