【摘要】 [目的]比较杉木涩籽形成过程中类黄酮类代谢物的积累特征，为进一步揭示杉木涩籽形成机制提供参考。[方法]以涩籽率分别为24%和90%的杉木家系(L家系和H家系)种子为试材，分别于授粉后70、80、90、100、110 d取样。采用试剂盒、高效液相色谱、荧光定量PCR等方式测定并分析不同家系种子类黄酮生物合成途径关键代谢物含量、酶活性和基因表达量。[结果]H家系种子发育过程中总黄酮含量及花青素、槲皮素、山奈酚、杨梅素和儿茶素等5种类黄酮类代谢物含量明显高于L家系；2个家系间查尔酮合成酶、查尔酮异构酶和二氢黄酮醇4-还原酶等3种酶活性仅在授粉后70 d呈现明显差异；H家系黄酮醇合成酶、无色花青素还原酶的活性水平变化趋势相近，且分别在授粉后80～110 d、70～110 d极显著高于L家系；2个家系种子CHS、CHI、LAR、ANR基因的相对表达量总体随时间变化呈逐渐升高趋势，且H家系低于L家系。[结论]在涩籽发育初期，类黄酮类次生代谢物质出现异常积累。杉木授粉后小孢子异常发育或与涩籽形成有关，涩籽种胚内细胞的应激系统处于激活状态。更多还原

【Abstract】 [Purpose] The difference of flavonoid accumulation features between astringent and germinant seeds were compared to reveal the mechanism of astringent seed development. [Method] Seeds from two families of Chinses fir with different seed astringent rates(H family and L family) were collected and sampled in 70, 80, 90, 100, 110 d after pollination respectively. Key metabolites contents, enzymes activities and genes expression levels inflavonoid biosynthetic pathway were assayed and analyzed by assay kit, HPLC and fluoro genic quantitative PCR. [Result] Contents of total flavonoid and flavonoid metabolites including anthocyanin, quercetin, kaempferol, myricetin and catechin in H family seeds were significantly higher than those in L family during development. The activities of chalcone synthase, chalcone isomerase and dihydroflavonol 4-reductase showed noticeable difference only in the 70 d after pollination between the two families. The activities of flavonol synthase and leuco anthocyanidin reductase in H family showed similar changing trends, and were significantly higher than those in L family in the period of 80-110 d and 70-110 d respectively after pollination. The expression levels of CHS, CHI, LAR, ANR genes in two families were observed to have a gradually increasing trend and gene expression levels in H family were lower than L family. [Conclusion] In the preliminary stage of astringent seed development, abnormal accumulation of secondary metabolites of flavonoids were observed. The abnormal development of microspores might be related to the formation of astringent seed after pollination, in addition the stress response system of germ cell was active in astringent seeds.更多还原