【摘要】 建立一种QuEChERS前处理方法结合高效液相色谱-串联质谱（high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS）技术同时检测荔枝龙眼基质中苯醚甲环唑、氯虫苯甲酰胺、溴氰虫酰胺及代谢物J9Z38残留量的分析方法。荔枝龙眼基质样品采用乙腈作为提取溶剂，利用50 mg十八烷基键合硅胶（C18）、50 mg N-丙基乙二胺（PSA）和10 mg纳米氧化锆（Nano-ZrO₂）3种吸附剂联合净化，用75 mm Infinity Lab Poroshell 120 SB-C18色谱柱进行分离，并以0.1%（体积分数）甲酸水溶液-乙腈为流动相进行梯度洗脱，多反应离子监测模式（MRM）扫描，以基质匹配标准溶液进行定量分析。结果显示：4种目标化合物在1～100μg/kg质量浓度范围内线性相关系数为0.9896～0.9999，定量限（LOQ）为0.10～10μg/kg。荔枝龙眼中4种目标分析物的加标回收率为82.48%～99.17%，日内和日间相对标准偏差分别为4.11%～7.77%、6.10%～13.27%。本方法具有易于操作、简便快速和灵敏度高等优势，可应用于荔枝龙眼实际样品中苯醚甲环唑、氯虫苯甲酰胺、溴氰虫酰胺及代谢物J9Z38残留的同时检测分析。更多还原

【Abstract】 An analytical method was established for the determination of difenoconazole,chlorantraniliprole,cyantraniliprole and their metabolite J9Z38 residues in litchi and longan by QuEChERS pretreatment method with high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） technique.Residues in litchi and longan samples were extracted with acetonitrile,purified with 50 mg C₁₈,50 mg PSA,and 10 mg Nano-ZrO₂,and then detected by HPLC-MS/MS with separation column of 75 mm Infinity Lab Poroshell 120 SB-C₁₈,and 0.1% formic acid-acetonitrile as mobile phase with gradient elution under multi-reactive ion monitoring（MRM） and quantified by external matrix-matched standard.Good linearities for the four analytes in the range of 1～100 μg/kg were obtained and the correlation coefficients（r²） were from 0.9896 to 0.9999.The limits of quantitation（LOQs） were among 0.10 to 10 μg/kg.The recoveries in the litchi and longan were ranged from 82.48% to 99.17%,the intra-day precisions and inter-day precisions were 4.11%～7.77% and 6.10%～13.27%,respectively.The developed method have several advantages:easy to operate,simple and rapid,and high sensitivity.The method can be used for monitoring of difenoconazole,chlorantraniliprole,cyantraniliprole and their metabolite J9 Z38 in real litchi and longan samples.更多还原